Characteristics of paclitaxel-loaded chitosan oligosaccharide nanoparticles and their preparation by interfacial polyaddition in O/W miniemulsion system

被引：16

作者：

Du, Yong-Zhong ^{[1
]}

Wang, Ling ^{[1
]}

Dong, Ying ^{[1
]}

Yuan, Hong ^{[1
]}

Hu, Fu-Qiang ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 79卷 / 04期

关键词：

Chitosan oligosaccharide; Ethylene glycol diglycidyl ether; Interfacial polyaddition; Nanoparticles; Paclitaxel; Drug entrapment efficiency; In vitro drug release; IN-VITRO; GENE DELIVERY; PHASE-I; MICELLES; CYTOTOXICITY; SOLUBILITY; COMPLEXES; LIPOSOME; EMULSION;

D O I：

10.1016/j.carbpol.2009.10.032

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Paclitaxel-loaded chitosan oligosaccharide (CSO) nanoparticles were prepared by interfacial polyaddition between amino group of CSO and epoxy group of ethylene glycol diglycidyl ether (EGDE) in an O/W mini-emulsion system. Using Span 85 and Tween 20 as the surfactants of oil phase and water phase, respectively, the stable O/W miniemulsion with about 200 nm droplet size could be obtained. When the molar ratio of EGDE to CSO was increased from 2 to 6, the average size of the obtained nanoparticles increased from 156.2 to 218.9 nm, the drug entrapment efficiency was increased from 83.68% to 92.30%, and the paclitaxel release rate was slowed. Fixing the molar ratio of EGDE to CSO at 4, the average size of prepared nanoparticles decreased from 282.1 to 140.4 nm when the total amount of CSO and EGDE was increased, while the drug entrapment efficiency increased, and the drug release rate in vitro decreased. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1034 / 1039

页数：6

共 32 条

[11] Uptake of FITC-chitosan nanoparticles by a549 cells [J].

Huang, M ;

Ma, ZS ;

Khor, E ;

Lim, LY .

PHARMACEUTICAL RESEARCH, 2002, 19 (10) :1488-1494

[12] Preparation and pharmacodynamics of low-molecular-weight chitosan nanoparticles containing insulin [J].

Huang, Xuan ;

Du, Yong-Zhong ;

Yuan, Hong ;

Hu, Fu-Qiang .

CARBOHYDRATE POLYMERS, 2009, 76 (03) :368-373

[13] Hydrotropic polymer micelle system for delivery of paclitaxel [J].

Huh, KM ;

Lee, SC ;

Cho, YW ;

Lee, JW ;

Jeong, JH ;

Park, K .

JOURNAL OF CONTROLLED RELEASE, 2005, 101 (1-3) :59-68

[14] Chitosan as a novel nasal delivery system for vaccines [J].

Illum, L ;

Jabbal-Gill, I ;

Hinchcliffe, M ;

Fisher, AN ;

Davis, SS .

ADVANCED DRUG DELIVERY REVIEWS, 2001, 51 (1-3) :81-96

[15] Development of nonionic sulfactant/phospholipid o/w emulsion as a paclitaxel delivery system [J].

Kan, P ;

Chen, ZB ;

Lee, CJ ;

Chu, IM .

JOURNAL OF CONTROLLED RELEASE, 1999, 58 (03) :271-278

[16] Trimethylated chitosans as non-viral gene delivery vectors: Cytotoxicity and transfection efficiency [J].

Kean, T ;

Roth, S ;

Thanou, M .

JOURNAL OF CONTROLLED RELEASE, 2005, 103 (03) :643-653

[17] Hydrotropic polymeric micelles for enhanced paclitaxel solubility: In vitro and in vivo characterization [J].

Lee, Sang Cheon ;

Huh, Kang Moo ;

Lee, Jaehwi ;

Cho, Yong Woo ;

Galinsky, Raymond E. ;

Park, Kinam .

BIOMACROMOLECULES, 2007, 8 (01) :202-208

[18] Antitumor drug Paclitaxel-loaded pH-sensitive nanoparticles targeting tumor extracellular pH [J].

Li, Fan ;

Wu, Hong ;

Zhang, Hui ;

Li, Fei ;

Gu, Chun-hu ;

Yang, Qian .

CARBOHYDRATE POLYMERS, 2009, 77 (04) :773-778

[19] Inclusion complexes of paclitaxel and oligo(ethylenediamino) bridged bis(β-cyclodextrin)s:: solubilization and antitumor activity [J].

Liu, Y ;

Chen, GS ;

Chen, Y ;

Cao, DX ;

Ge, ZQ ;

Yuan, YJ .

BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (22) :5767-5775

[20] Mucoadhesive polymers in peroral peptide drug delivery .4. Polycarbophil and chitosan are potent enhancers of peptide transport across intestinal mucosae in vitro [J].

Luessen, HL ;

Rentel, CO ;

Kotze, AF ;

Lehr, CM ;

deBoer, AG ;

Verhoef, JC ;

Junginger, HE .

JOURNAL OF CONTROLLED RELEASE, 1997, 45 (01) :15-23

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