Bioguided extraction of phenolic compounds and UHPLC-ESI-Q-TOF-MS/MS characterization of extracts of Moringa oleifera leaves collected in Brazil

被引：66
作者：
Cadorin Oldoni, Tatiane Luiza [1 ]
Merlin, Nathalie [1 ]
Karling, Marieli [1 ]
Carpes, Solange Teresinha [1 ]
de Alencar, Severino Matias [2 ]
Ferreira Morales, Rafael Gustavo [3 ]
da Silva, Evandro Aparecido [4 ]
Pilau, Eduardo Jorge [4 ]
机构：
[1] Fed Technol Univ Parana UTFPR, Dept Chem, BR-85503390 Pato Branco, PR, Brazil
[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, BR-13418900 Piracicaba, SP, Brazil
[3] Empresa Pesquisa Agr & Extensao Rural Santa Catar, BR-88318112 Itajai, SC, Brazil
[4] State Univ Maringa UEM, Dept Chem, Av Colombo,5790 Jardim Univ,CP 591, BR-87020900 Maringa, Parana, Brazil
来源：
FOOD RESEARCH INTERNATIONAL | 2019年 / 125卷
关键词：
Bioguided purification; Natural products; Chromatography; Chemical composition; Phenolic compounds; Antioxidant activity; ANTIOXIDANT ACTIVITY; BIOACTIVE COMPOUNDS; MULTIPURPOSE TREE; IN-VITRO; PHYTOCHEMICALS; FLAVONOIDS; NUTRIENTS; PLANTS; L;
D O I：
10.1016/j.foodres.2019.108647
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Moringa oleifera leaves are used in Brazilian folk medicine for their hypoglycemic and nutritional properties. In this context, the chemical and biological characteristics were determined. Conventional successive solid-liquid extraction with simultaneous bioguided purification using solvents with different polarities was performed with M. oleifera leaves, yielding six fractions and extracts. The fractions showed better results for antioxidant activity than the extracts. All of them were evaluated by scavenging of synthetic free radicals and reactive oxygen species, and Fr-Ace and Fr-EtOAc showed > 100 mg GAE g(-1) of phenolic content, while for FRAP and ORAC assays the values were higher than 1600 mu mol Fe2+ g(-1) and 3500 mmol TEAC g(-1) respectively. The UPLC-ESI-QTOF-MS analysis of hydroalcoholic extract (HE) allowed identifying 24 compounds, with flavonoid derivatives being the most abundant group. Furthermore, the alkaloid trigonelline and sesquiterpenoid abscisic acid were identified for the first time in M. oleifera leaves. Finally, gallic acid, caffeic acid, rutin and quercetin were found in concentrations of 16.5, 2129, 477.4 and 127.5 mu g g(-1) respectively in HE, all of which were higher in fractions and extracts. These results suggest that bioguided extraction is an important technique, due to its ability to concentrate active compounds in a logical and rational way. In addition, M. oleifera leaves grown in Brazil are an important source of phenolic compounds with antioxidant activity that can be used in food, nutraceutical and pharmaceutical products.
引用
收藏
页数：9
相关论文
共 46 条
[1]   Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana [J].
Amaglo, Newton K. ;
Bennett, Richard N. ;
Lo Curto, Rosario B. ;
Rosa, Eduardo A. S. ;
Lo Turco, Vincenzo ;
Giuffrida, Angela ;
Lo Curto, Alberto ;
Crea, Francesco ;
Timpo, Gladys M. .
FOOD CHEMISTRY, 2010, 122 (04) :1047-1054
[2]   Studies on neuropharmacological profile of ethanol extract of Moringa oleifera leaves in mice [J].
Bakre, Adewale G. ;
Aderibigbe, Adegbuyi O. ;
Ademowo, Olusegun G. .
JOURNAL OF ETHNOPHARMACOLOGY, 2013, 149 (03) :783-789
[3]   Effect of solvent evaporation method on phenolic compounds and the antioxidant activity of Moringa oleifera cultivated in Southern Tunisia [J].
Bennour, N. ;
Mighri, H. ;
Eljani, H. ;
Zammouri, T. ;
Akrout, A. .
SOUTH AFRICAN JOURNAL OF BOTANY, 2020, 129 :181-190
[4]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[5]  
BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25
[6]   Isolation and characterization of bioactive compounds from plant resources: The role of analysis in the ethnopharmacological approach [J].
Brusotti, G. ;
Cesari, I. ;
Dentamaro, A. ;
Caccialanza, G. ;
Massolini, G. .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2014, 87 :218-228
[7]   Impact of extraction techniques on antioxidant capacities and phytochemical composition of polyphenol-rich extracts [J].
Castro-Lopez, Cecilia ;
Ventura-Sobrevilla, Janeth M. ;
Gonzalez-Hernandez, Maria D. ;
Rojas, Romeo ;
Ascacio-Valdes, Juan A. ;
Aguilar, Cristobal N. ;
Martinez-Avila, Guillermo C. G. .
FOOD CHEMISTRY, 2017, 237 :1139-1148
[8]   Cytotoxic and antioxidant activities of Macfadyena unguis-cati L. aerial parts and bioguided isolation of the antitumor active components [J].
Chen, Lianghua ;
Chen, Di ;
Zheng, Zhizhong ;
Liu, Shaosong ;
Tong, Qingxuan ;
Xiao, Jianbo ;
Lin, Hetong ;
Ming, Yanlin .
INDUSTRIAL CROPS AND PRODUCTS, 2017, 107 :531-538
[9]   Phenolic compound contents and antioxidant activity in plants with nutritional and/or medicinal properties from the Peruvian Andean region [J].
Chirinos, Rosana ;
Pedreschi, Romina ;
Rogez, Herve ;
Larondelle, Yvan ;
Campos, David .
INDUSTRIAL CROPS AND PRODUCTS, 2013, 47 :145-152
[10]   In vitro scavenging capacity of annatto seed extracts against reactive oxygen and nitrogen species [J].
Chiste, Renan Campos ;
Mercadante, Adriana Zerlotti ;
Gomes, Ana ;
Fernandes, Eduarda ;
Fontes da Costa Lima, Jose Luis ;
Bragagnolo, Neura .
FOOD CHEMISTRY, 2011, 127 (02) :419-426
12345