2009 Pandemic H1N1 Influenza Virus Replicates in Human Lung Tissues

被引：13
作者：
Zhang, Jinxia [1 ,2 ,3 ]
Zhang, Zengfeng [5 ]
Fan, Xiaohui [5 ]
Liu, Yuansheng [3 ,4 ]
Wang, Jia [1 ,2 ,3 ]
Zheng, Zuoyi [1 ,2 ]
Chen, Rirong [1 ,2 ]
Wang, Pui [1 ,2 ]
Song, Wenjun [1 ,2 ]
Chen, Honglin [1 ,2 ,3 ]
Guan, Yi [1 ,2 ,3 ]
机构：
[1] Univ Hong Kong, State Key Lab Emerging Infect Dis, Dept Microbiol, Li Ka Shing Fac Med, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, State Key Lab Emerging Infect Dis, Res Ctr Infect & Immunol, Li Ka Shing Fac Med, Hong Kong, Hong Kong, Peoples R China
[3] Shantou Univ, Coll Med, Int Inst Infect & Immun, Shantou, Guangdong, Peoples R China
[4] Shantou Univ, Coll Med, Dept Med, Affiliated Hosp 1, Shantou, Guangdong, Peoples R China
[5] Guangxi Med Univ, Dept Pathol, Guangxi, Peoples R China
来源：
JOURNAL OF INFECTIOUS DISEASES | 2010年 / 201卷 / 10期
基金：
美国国家卫生研究院;
关键词：
SWINE; INFECTION; TRANSMISSION; PATHOGENESIS; FERRETS;
D O I：
10.1086/650544
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The replication activity of 2009 pandemic H1N1 influenza virus in human lung cells was evaluated in this study. Twenty-two surgically removed human lung tissue samples were infected ex vivo with pandemic H1N1 influenza virus (A/California/04/2009), seasonal human H1N1 influenza virus (A/Shantou/92/09), or a highly pathogenic H5N1 influenza virus (A/Vietnam/1194/04). Examination of nucleoprotein expression and viral RNA replication in the infected human lung tissue samples showed that whereas the replication of pandemic H1N1 influenza virus varied between tissue samples, overall this virus replicated more efficiently than seasonal H1N1 influenza virus but less efficiently than H5N1 influenza virus. Double-immunostaining for viral antigens and cellular markers indicated that pandemic H1N1 influenza virus replicates in type 2 alveolar epithelial cells.
引用
收藏
页码：1522 / 1526
页数：5
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