Genotyping of Trypanosoma cruzi: Systematic Selection of Assays Allowing Rapid and Accurate Discrimination of All Known Lineages

被引：104
作者：
Lewis, Michael D. [1 ]
Ma, Jonathan [2 ]
Yeo, Matthew [1 ]
Carrasco, Hernan J. [3 ]
Llewellyn, Martin S. [1 ]
Miles, Michael A. [1 ]
机构：
[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, Pathogen Mol Biol Unit, London WC1E 7HT, England
[2] Biocanc Treatment Int Ltd, Bioinformat Ctr, Shatin, Hong Kong, Peoples R China
[3] Cent Univ Venezuela, Fac Med, Inst Trop Med, Caracas, Venezuela
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2009年 / 81卷 / 06期
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
SUBUNIT RIBOSOMAL-RNA; CHAGAS-DISEASE; GENETIC EXCHANGE; MINI-EXON; PHYLOGENETIC LINEAGES; NUCLEOTIDE-SEQUENCES; POPULATION-STRUCTURE; TRIATOMA-INFESTANS; RECOMBINATION; EPIDEMIOLOGY;
D O I：
10.4269/ajtmh.2009.09-0305
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic markers are available to determine the DTU to which a T. cruzi isolate belongs, but there is no consensus on which should be used. We selected five assays: three polymerase chain reaction (PCR)-restriction fragment length polymorphisms based oil single nucleotide polymorphisms (SNPs) in the HSP60, Histone H1, and GPI loci, and PCR product size polymorphism of the LSU rDNA and mini-exon loci. Each assay was tested for its capacity to differentiate between DTUs using a panel of 48 genetically diverse T cruzi clones. Some markers allowed unequivocal identification of individual DTUs, however, only by using a combination of multiple markers Could all six DTUs be resolved. Based upon the results we recommend a triple-assay comprising the LSU rDNA, HSP60 and GPI markers for reliable, rapid, low-cost DTU assignment.
引用
收藏
页码：1041 / 1049
页数：9
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