Genotyping of Trypanosoma cruzi: Systematic Selection of Assays Allowing Rapid and Accurate Discrimination of All Known Lineages

被引：104

作者：

Lewis, Michael D. ^{[1
]}

Ma, Jonathan ^{[2
]}

Yeo, Matthew ^{[1
]}

Carrasco, Hernan J. ^{[3
]}

Llewellyn, Martin S. ^{[1
]}

Miles, Michael A. ^{[1
]}

机构：

[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, Pathogen Mol Biol Unit, London WC1E 7HT, England

[2] Biocanc Treatment Int Ltd, Bioinformat Ctr, Shatin, Hong Kong, Peoples R China

[3] Cent Univ Venezuela, Fac Med, Inst Trop Med, Caracas, Venezuela

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2009年 / 81卷 / 06期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

SUBUNIT RIBOSOMAL-RNA; CHAGAS-DISEASE; GENETIC EXCHANGE; MINI-EXON; PHYLOGENETIC LINEAGES; NUCLEOTIDE-SEQUENCES; POPULATION-STRUCTURE; TRIATOMA-INFESTANS; RECOMBINATION; EPIDEMIOLOGY;

D O I：

10.4269/ajtmh.2009.09-0305

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic markers are available to determine the DTU to which a T. cruzi isolate belongs, but there is no consensus on which should be used. We selected five assays: three polymerase chain reaction (PCR)-restriction fragment length polymorphisms based oil single nucleotide polymorphisms (SNPs) in the HSP60, Histone H1, and GPI loci, and PCR product size polymorphism of the LSU rDNA and mini-exon loci. Each assay was tested for its capacity to differentiate between DTUs using a panel of 48 genetically diverse T cruzi clones. Some markers allowed unequivocal identification of individual DTUs, however, only by using a combination of multiple markers Could all six DTUs be resolved. Based upon the results we recommend a triple-assay comprising the LSU rDNA, HSP60 and GPI markers for reliable, rapid, low-cost DTU assignment.

引用

页码：1041 / 1049

页数：9

共 52 条

[1]

[Anonymous], 2002, WORLD HLTH REP

[2] Trypanosoma cruzi:: A considerable phylogenetic divergence indicates that the agent of Chagas disease is indigenous to the native fauna of the United States [J].

Barnabé, C ;

Yaeger, R ;

Pung, O ;

Tibayrenc, M .

EXPERIMENTAL PARASITOLOGY, 2001, 99 (02) :73-79

[3] Trypanosoma cruzi: Study of the distribution of two widespread clonal genotypes in Bolivian Triatoma infestans vectors shows a high frequency of mixed infections [J].

Bosseno, MF ;

Telleria, J ;

Vargas, F ;

Yaksic, N ;

Noireau, F ;

Morin, A ;

Breniere, SF .

EXPERIMENTAL PARASITOLOGY, 1996, 83 (03) :275-282

[4] Just one cross appears capable of dramatically altering the population biology of a eukaryotic pathogen like Toxoplasma gondii [J].

Boyle, Jon P. ;

Rajasekar, Badri ;

Saeij, Jeroen P. J. ;

Ajiokat, James W. ;

Berriman, Matthew ;

Paulsen, Ian ;

Roos, David S. ;

Sibley, L. David ;

White, Michael W. ;

Boothroyd, John C. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (27) :10514-10519

[5] Characterisation of large and small subunit rRNA and mini-exon genes further supports the distinction of six Trypanosoma cruzi lineages [J].

Brisse, S ;

Verhoef, J ;

Tibayrenc, M .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2001, 31 (11) :1218-1226

[6] Identification of six Trypanosoma cruzi phylogenetic lineages by random amplified polymorphic DNA and multilocus enzyme electrophoresis [J].

Brisse, S ;

Barnabé, C ;

Tibayrenc, M .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2000, 30 (01) :35-44

[7] Evidence for genetic exchange and hybridization in Trypanosoma cruzi based on nucleotide sequences and molecular karyotype [J].

Brisse, Sylvain ;

Henriksson, Jan ;

Barnabe, Christian ;

Douzery, Emmanuel J. P. ;

Berkvens, Dirk ;

Serrano, Myrna ;

De Carvalho, Maria Ruth C. ;

Buck, Gregory A. ;

Dujardin, Jean-Claude ;

Tibayrenc, Michel .

INFECTION GENETICS AND EVOLUTION, 2003, 2 (03) :173-183

[8] Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease [J].

Burgos, Juan M. ;

Altcheh, Jaime ;

Bisio, Margarita ;

Duffy, Tomas ;

Valadares, Helder M. S. ;

Seidenstein, Marfa Elena ;

Piccinali, Romina ;

Freitas, Jorge M. ;

Levin, Mariano J. ;

Macchi, Liliana ;

Macedo, Andrea M. ;

Freilij, Hector ;

Schijman, Alejandro G. .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2007, 37 (12) :1319-1327

[9] The determinants of Chagas disease: Connecting parasite and host genetics [J].

Campbell, DA ;

Westenberger, SJ ;

Sturm, NR .

CURRENT MOLECULAR MEDICINE, 2004, 4 (06) :549-562

[10] Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina [J].

Cardinal, Marta V. ;

Lauricella, Marta A. ;

Ceballos, Leonardo A. ;

Lanati, Leonardo ;

Marcet, Paula L. ;

Levin, Mariano J. ;

Kitron, Uriel ;

Guertler, Ricardo E. ;

Schijman, Alejandro G. .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2008, 38 (13) :1533-1543

← 1 2 3 4 5 6 →