Bone marrow derived mesenchymal stem cells alleviated brain injury via down-regulation of interleukin-1β in focal cerebral ischemic rats

被引：0

作者：

Zhao, Yansong ^{[1
]}

Wang, Xiaoli ^{[2
]}

Dong, Peng ^{[2
]}

Xu, Qinyan ^{[2
]}

Ma, Ze ^{[2
]}

Mu, Qingjie ^{[3
]}

Sun, Xihe ^{[2
]}

Jiang, Zhengchen ^{[4
]}

Wang, Xin ^{[5
]}

机构：

[1] Weifang Med Univ, Affiliated Hosp, Inst Clin Med, Dept Ophthalmol, Weifang, Shandong, Peoples R China

[2] Weifang Med Univ, Dept Med Imaging, Weifang, Shandong, Peoples R China

[3] Weifang Med Univ, Inst Clin Med, Dept Hematol, Weifang, Shandong, Peoples R China

[4] Weifang Med Univ, Clin Med Inst, Weifang, Shandong, Peoples R China

[5] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Neurosurg, Boston, MA 02115 USA

来源：

AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2016年 / 8卷 / 03期

基金：

比尔及梅琳达.盖茨基金会; 中国国家自然科学基金;

关键词：

Bone marrow-derived mesenchymal stem cells; brain edema; cerebral infarction; interleukin-1; beta; cerebral ischemia; rats; NF-KAPPA-B; ARTERY OCCLUSION; STROKE; NEUROPROTECTION; MICE; INFLAMMATION; EXPRESSION; PATHWAY; MODEL;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Interleukin-1 beta (IL-1 beta) plays an important role in brain injury after focal ischemia, and bone marrow-derived mesenchymal stem cells (BMSCs) are capable of reducing the expression of IL-18, we investigated the effects of BMSCs transplantation on brain edema and cerebral infarction as well as the underlying mechanisms via IL -113. Male Sprague-Dawley rats were randomly divided into five groups: Normal + phosphate-buffered saline (PBS), middle cerebral artery occlusion (MCAO) + PBS, Normal + BMSCs, MCAO + BMSCs and MCAO + |L-1ra (an antagonist of IL -113). BMSCs were transplanted 24 hours after MCAO, and brain edema was evaluated by Magnetic Resonance Imaging (MRI) and brain water content method after BMSCs transplantation. The expression of NeuN and AQP4 was analyzed by immunofluorescence staining. Protein level of AQP4 and IL -113 was detected by western blot analysis 48 hours after transplantation. The results showed that BMSCs transplantation reduced brain edema by measurement of brain water content and ADC Value of MRI, as well as the expression of AQP4 and IL-1 beta. It was also found that BMSCs transplantation could alleviate the cerebral infarction volume and neuronal damage. Both the brain edema and the cerebral infarction were associated with IL-1 beta expression. In conclusion, BMSCs transplantation was capable of alleviating brain edema as well as reducing cerebral infarction via down-regulation of IL-1 beta expression, thus repair the injured brain in focal cerebral ischemic rats.

引用

页码：1541 / 1550

页数：10

共 18 条

[11]

Pandya Rachna S., 2011, Central Nervous System Agents in Medicinal Chemistry, V11, P81

[12] Mesenchymal stem cells stabilize the blood-brain barrier through regulation of astrocytes [J].

Park, Hyun Jung ;

Shin, Jin Young ;

Kim, Ha Na ;

Oh, Se Hee ;

Song, Sook K. ;

Lee, Phil Hyu .

STEM CELL RESEARCH & THERAPY, 2015, 6

[13] Regulation of IL-1β-induced NF-κB by hydroxylases links key hypoxic and inflammatory signaling pathways [J].

Scholz, Carsten C. ;

Cavadas, Miguel A. S. ;

Tambuwala, Murtaza M. ;

Hams, Emily ;

Rodriguez, Javier ;

von Kriegsheim, Alex ;

Cotter, Philip ;

Bruning, Ulrike ;

Fallon, Padraic G. ;

Cheong, Alex ;

Cummins, Eoin P. ;

Taylor, Cormac T. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (46) :18490-18495

[14]

Sun BZ, 2014, INT J CLIN EXP MED, V7, P3087

[15] Human Mesenchymal Stem Cell Transplantation Changes Proinflammatory Gene Expression Through a Nuclear Factor-κB-Dependent Pathway in a Rat Focal Cerebral Ischemic Model [J].

Wang, Hui ;

Nagai, Atsushi ;

Sheikh, Abdullah Md. ;

Liang, Xue Yun ;

Yano, Shozo ;

Mitaki, Shingo ;

Ishibashi, Yutaka ;

Kobayashi, Shotai ;

Kim, Seung U. ;

Yamaguchi, Shuhei .

JOURNAL OF NEUROSCIENCE RESEARCH, 2013, 91 (11) :1440-1449

[16] Methazolamide and Melatonin Inhibit Mitochondrial Cytochrome C Release and Are Neuroprotective in Experimental Models of Ischemic Injury [J].

Wang, Xin ;

Figueroa, Bryan E. ;

Stavrovskaya, Irina G. ;

Zhang, Yi ;

Sirianni, Ana C. ;

Zhu, Shan ;

Day, Arthur L. ;

Kristal, Bruce S. ;

Friedlander, Robert M. .

STROKE, 2009, 40 (05) :1877-1885

[17] Rapid health transition in China, 1990-2010: findings from the Global Burden of Disease Study 2010 [J].

Yang, Gonghuan ;

Wang, Yu ;

Zeng, Yixin ;

Gao, George F. ;

Liang, Xiaofeng ;

Zhou, Maigeng ;

Wan, Xia ;

Yu, Shicheng ;

Jiang, Yuhong ;

Naghavi, Mohsen ;

Vos, Theo ;

Wang, Haidong ;

Lopez, Alan D. ;

Murray, Christopher J. L. .

LANCET, 2013, 381 (9882) :1987-2015

[18] Neuroprotective Effects of (-)-Epigallocatechin-3-Gallate Against Focal Cerebral Ischemia/Reperfusion Injury in Rats Through Attenuation of Inflammation [J].

Zhang, FengJin ;

Li, Na ;

Jiang, LinLan ;

Chen, LongHao ;

Huang, MuTu .

NEUROCHEMICAL RESEARCH, 2015, 40 (08) :1691-1698

← 1 2 →