The Puf-A Protein Is Required for Primordial Germ Cell Development

被引：3

作者：

Ko, Chi-Fong ^{[1
]}

Chang, Yi-Chieh ^{[1
]}

Cho, Huan-Chieh ^{[1
]}

Yu, John ^{[1
]}

机构：

[1] Chang Gung Mem Hosp, Inst Stem Cell & Translat Canc Res, Taoyuan 333, Taiwan

来源：

CELLS | 2022年 / 11卷 / 09期

关键词：

Puf-A; nucleophosmin (NPM1); p53; primordial germ cells (PGCs); proliferation; apoptosis; PGC maintenance; GENE; FAMILY; BLIMP1; EXPRESSION; INTERACTS; LINEAGE; LIN28; NOP9; PUM1; P53;

D O I：

10.3390/cells11091476

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Puf-A, a nucleolar Puf domain protein, is required for ribosome biogenesis. A study of Puf-A in zebrafish has shown that Puf-A is highly expressed in primordial germ cells (PGCs) and participates in PGC development. However, it remains unclear how Puf-A governs PGC development in mammals. Here, we generated transgenic mice carrying inducible Puf-A shRNA and obtained double heterozygous mice with Puf-A shRNA and Oct4-EGFP to examine the behavior of PGCs. It was found that the knockdown of Puf-A led to the loss of a considerable number of PGCs and a slowdown of the movement of the remaining PGCs. Puf-A and NPM1 colocalized in clusters in the nuclei of the PGCs. The silencing of Puf-A resulted in the translocation of NPM1 from nucleolus to nucleoplasm and the hyperactivation of p53 in the PGCs. The PGCs in Puf-A knockdown embryos showed a significant increase in subpopulations of PGCs at G1 arrest and apoptosis. Moreover, the expression of essential genes associated with PGC maintenance was decreased in the Puf-A knockdown PGCs. Our study showed that Puf-A governed PGC development by regulating the growth, survival, and maintenance of PGCs. We also observed the alterations of NPM1 and p53 upon Puf-A knockdown to be consistent with the previous study in cancer cells, which might explain the molecular mechanism for the role of Puf-A in PGC development.

引用

页数：19

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