Spectrophotometric Determination of Thiocyanate in Human Saliva Employing Micropumping Multicommutation Flow System

被引：14

作者：

Silva Junior, Joilton J. ^{[1
]}

Farias, Marcelo A. ^{[1
]}

Silva, Valdinete L. ^{[2
]}

Montenegro, Maria C. B. S. M. ^{[3
]}

Araujo, Alberto N. ^{[3
]}

Lavorante, Andre F. ^{[4
]}

Paim, Ana Paula S. ^{[1
]}

机构：

[1] Univ Fed Pernambuco, Dept Quim Fundamental, BR-50740550 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Dept Engn Quim, BR-50740550 Recife, PE, Brazil

[3] Univ Porto, Fac Farm, REQUIMTE, Dept Quim Fis, P-4100 Oporto, Portugal

[4] Univ Fed Pernambuco, Dept Quim, BR-50740550 Recife, PE, Brazil

来源：

SPECTROSCOPY LETTERS | 2010年 / 43卷 / 03期

关键词：

human saliva; multicommutation flow analysis; multipumping; spectrophotometry; thiocyanate; CYANIDE; SAMPLES; IONS; INSTRUMENTATION; CHROMATOGRAPHY; URINE; WATER; ACID;

D O I：

10.1080/00387010903287094

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A procedure based on a multicommutated approach for the determination of thiocyanate (SCN-) in human saliva is described. The method was based on the reaction of Fe(III) with SCN-, producing a red complex that was monitored at 480nm. Under optimum experimental conditions, a linear response ranging from 0.5 to 10.0mmolL-1 SCN- (R=0.9991), a relative standard deviation of 1.0% (n=10) for a sample 5mmolL-1 SCN-, a detection limit (3 sigma criterion) of 50 mu molL-1, a sampling rate of 60 determinations per hour, reagent consumption of 0.02mg Fe(III) per determination, and a waste generation of 1.6mL per determination were achieved.

引用

页码：213 / 219

页数：7

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