LncRNA NEAT1 facilitates the progression of sepsis through up-regulating TSP-1 via sponging miR-370-3p

被引:26
作者
Wu, X-Y [1 ]
Fang, Y. [2 ]
Zheng, F-X [3 ]
Zhang, Y-Z [1 ]
Li, Q-L [4 ]
机构
[1] Danzhou Peoples Hosp, Dept Gen Surg, Danzhou, Hainan, Peoples R China
[2] Hubei Coll Chinese Med, Dept Clin Med, Jingzhou, Peoples R China
[3] Shanxi Med Univ, Hosp 1, Dept Resp & Crit Care Med, Taiyuan, Shanxi, Peoples R China
[4] Shengli Oilfield Ctr Hosp, Dept Burn & Plast Surg, Dongying, Shandong, Peoples R China
关键词
Sepsis; LncRNA NEAT1; MiR-370-3p; TSP-1; Inflammation; Proliferation; Apoptosis; LONG NONCODING RNA; CARCINOMA; APOPTOSIS; CANCER; PROLIFERATION; EXPRESSION;
D O I
10.26355/eurrev_202001_19931
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
OBJECTIVE: Sepsis is a systemic inflammatory disease. LncRNA NEAT1 has been reported to be up-regulated in sepsis patients. Nevertheless, the modulatory network of NEAT1 in sepsis remains to be revealed. PATIENTS AND METHODS: The abundance of long noncoding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1), miR-370-3p, and thrombospondin-1 (TSP-1) were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) in sepsis patients and lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Enzyme-linked immunosorbent assay (ELISA) was performed to examine the concentration of cytokines in RAW 264.7 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry assay, and Western blot assay were conducted to detect the proliferation and apoptosis of RAW 264.7 cells. Dual-Luciferase reporter assay, RNA immunoprecipitation (RIP) assay, and RNA-pull down assay were conducted to confirm the combination between miR-370-3p and NEAT1 or TSP-1 in RAW 264.7 cells. RESULTS: The enrichment of NEAT1 was enhanced in sepsis patients and LPS-stimulated RAW 264.7 cells. NEAT1 contributed to LPS-induced inflammation and apoptosis of RAW 264.7 cells. MiR-370-3p bound to NEAT1, and it was negatively regulated by NEAT1 in RAW 264.7 cells. LPS promoted the inflammation and apoptosis while restrained the proliferation of RAW 264.7 cells via NEAT1/miR-370-3p axis. TSP-1 was a target of miR-370-3p in RAW 264.7 cells, and miR-370-3p suppressed the inflammation and apoptosis while it facilitated the proliferation of LPS-induced RAW 264.7 cells via TSP-1. CONCLUSIONS: LncRNA NEAT1 promoted the inflammation and apoptosis while restrained the proliferation of LPS-stimulated RAW 264.7 cells through the miR-370-3p/TSP-1 axis.
引用
收藏
页码:333 / 344
页数:12
相关论文
共 27 条
[1]   The functions of animal microRNAs [J].
Ambros, V .
NATURE, 2004, 431 (7006) :350-355
[2]   THROMBIN-SENSITIVE PROTEIN OF HUMAN PLATELET MEMBRANES [J].
BAENZIGE.NL ;
BRODIE, GN ;
MAJERUS, PW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (01) :240-+
[3]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[4]   MicroRNA signatures in human cancers [J].
Calin, George A. ;
Croce, Carlo M. .
NATURE REVIEWS CANCER, 2006, 6 (11) :857-866
[5]   A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA [J].
Cesana, Marcella ;
Cacchiarelli, Davide ;
Legnini, Ivano ;
Santini, Tiziana ;
Sthandier, Olga ;
Chinappi, Mauro ;
Tramontano, Anna ;
Bozzoni, Irene .
CELL, 2011, 147 (02) :358-369
[6]   Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway [J].
Chen, Fukun ;
Feng, Zhiping ;
Zhu, Jialun ;
Liu, Pengjie ;
Yang, Chuanzhou ;
Huang, Rongkai ;
Deng, Zhiyong .
CANCER BIOLOGY & THERAPY, 2018, 19 (12) :1139-1152
[7]  
Chen XJ, 2015, AM J CANCER RES, V5, P2808
[8]   Sepsis: a roadmap for future research [J].
Cohen, Jonathan ;
Vincent, Jean-Louis ;
Adhikari, Neill K. J. ;
Machado, Flavia R. ;
Angus, Derek C. ;
Calandra, Thierry ;
Jaton, Katia ;
Giulieri, Stefano ;
Delaloye, Julie ;
Opal, Steven ;
Tracey, Kevin ;
van der Poll, Tom ;
Pelfrene, Eric .
LANCET INFECTIOUS DISEASES, 2015, 15 (05) :581-614
[9]   miRNAs, cancer, and stem cell division [J].
Croce, CM ;
Calin, GA .
CELL, 2005, 122 (01) :6-7
[10]   Platelet function in septic multiple organ dysfunction syndrome [J].
Gawaz, M ;
Dickfeld, T ;
Bogner, C ;
FatehMoghadam, S ;
Neumann, FJ .
INTENSIVE CARE MEDICINE, 1997, 23 (04) :379-385