Cooperative action between L-Maf and Sox2 on δ-crystallin gene expression during chick lens development

被引:39
作者
Shimada, N [1 ]
Aya-Murata, T [1 ]
Reza, HM [1 ]
Yasuda, K [1 ]
机构
[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma, Nara 6300101, Japan
关键词
L-Maf; Sox2; lens development; lens induction; in ovo reporter assay; electroporation;
D O I
10.1016/S0925-4773(03)00002-9
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lens development is regulated by a variety of transcription factors with distinct properties. The lens-specific transcription factor, L-Maf, is essential for lens formation and induces lens-specific markers, such as the crystallin genes. In this study, we analyzed the mechanism by which L-Maf regulates delta-crystallin expression. Misexpression of L-Maf in the head ectoderm of lens placode-forming embryos by in ovo electroporation induced delta-crystallin only in the region surrounding the lens. To define this restricted expression, we misexpressed L-Maf together with other transcription factors implicated in delta-crystallin expression. Sox2 plus L-Maf expanded the delta-crystallin-inducible domain to the entire head ectoderm and simultaneously increased the quantity of delta-crystallin mRNA expressed. In contrast, co-expression of L-Maf with other factors such as Pax6, Six3 and Prox1 had little or no effect on delta-crystallin. We also observed that L-Maf and Sox2 cooperatively enhanced the transactivation of a reporter gene bearing the delta-crystallin enhancer in ovo, implying that L-Maf and Sox2 can: induce delta-crystallin through the same enhancer. In conclusion, we report here that L-Maf and Sox2 cooperatively regulate the expression of delta-crystallin during chick lens development. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:455 / 465
页数:11
相关论文
empty
未找到相关数据