Losses of the cyanobacterial toxin microcystin-LR from aqueous solution by adsorption during laboratory manipulations

被引：58

作者：

Hyenstrand, P ^{[1
]}

Metcalf, JS ^{[1
]}

Beattie, KA ^{[1
]}

Codd, GA ^{[1
]}

机构：

[1] Univ Dundee, Dept Biol Sci, Dundee DD1 4HN, Scotland

来源：

TOXICON | 2001年 / 39卷 / 04期

关键词：

microcystin-LR; adsorption; plastics; methanol;

D O I：

10.1016/S0041-0101(00)00168-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of plastic and methanol on the loss of microcystin-LR from solution was analysed by HPLC with photodiode array detection (HPLC-PDA). With plastic disposable pipette tips, the loss from an aqueous microcystin-LR (MC-LR) solution was 4.2% per lip operation. Using the same pipette tip, four operations were required to completely saturate a single tip with toxin. MC-LR attached to plastic pipette tips could subsequently be eluted by methanol and detected by HPLC-PDA. At methanol concentrations below 25% (v/v), recovered concentrations of MC-LR decreased significantly. Differences in MC-LR concentration were also noted by performing 50% dilution with Milli-Q water or methanol. The results are discussed in relation to the hydrophobicity of MC-LR, analytical procedures and the avoidance of toxin losses from solution during laboratory manipulations. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：589 / 594

页数：6

共 21 条

[1]

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[2]

BELL S.G., 1996, AGR CHEM ENV ISSUES, P109

[3] CYANOBACTERIA SECONDARY METABOLITES - THE CYANOTOXINS [J].