A practical toxicity bioassay for vicine and convicine levels in faba bean (Vicia faba)

被引:8
作者
Getachew, Fitsum [1 ]
Vandenberg, Albert [2 ]
Smits, Judit [1 ]
机构
[1] Univ Calgary, Dept Ecosyst & Publ Hlth, Calgary, AB, Canada
[2] Univ Saskatchewan, Ctr Crop Dev, Dept Plant Sci, Saskatoon, SK, Canada
关键词
favism; vicine-convicine; faba bean aglycones; BCNU-treated red blood cells; faba bean toxicity; glutathione depletion; G6PD DEFICIENT ERYTHROCYTES; RED-BLOOD-CELLS; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE DEFICIENCY; GLUTATHIONE; MEMBRANE; FAVISM; PROTEINS; FABABEANS; HEMOLYSIS; REMOVAL;
D O I
10.1002/jsfa.9049
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
BACKGROUNDFaba bean (Vicia faba) vicine and convicine (V-C) aglycones (divicine and isouramil respectively) provoke an acute hemolytic anemia called favism in individuals with a glucose-6-phosphate dehydrogenase (G6PD) enzyme defect in their red blood cells. Geneticists/plant breeders are working with faba bean to decrease V-C levels to improve public acceptance of this high-protein pulse crop. Here, we present a fast and simple ex vivo in vitro bioassay for V-C toxicity testing of faba bean or faba bean food products. RESULTSWe have shown that 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU)-treated (i.e., sensitized) normal red blood cells, like G6PD-defective blood, displayed (i) continuous glutathione (GSH) depletion with no regeneration as incubation time and the dose of aglycones increased, (ii) progressive accumulation of denatured hemoglobin products into high molecular weight (HMW) proteins with increased aglycone dose, (iii) both band 3 membrane proteins and hemichromes, in HMW protein aggregates. We have also demonstrated that sensitized red blood cells can effectively differentiate various levels of toxicity among faba bean varieties through the two hemolysis biomarkers: GSH depletion and HMW clumping. CONCLUSIONBCNU-sensitized red blood cells provide an ideal model for favism blood, to assess and compare the toxicity of faba bean varieties and their food products. (c) 2018 Society of Chemical Industry
引用
收藏
页码:5105 / 5111
页数:7
相关论文
共 31 条
[1]   Band 3/complement-mediated recognition and removal of normally senescent and pathological human erythrocytes [J].
Arese, P ;
Turrini, F ;
Schwarzer, E .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2005, 16 (4-6) :133-146
[2]  
ARESE P, 1990, SEMIN HEMATOL, V27, P1
[3]  
Arese P, 1980, PROG CLIN BIOL RES, V55, P725
[4]   Life and Death of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficient Erythrocytes - Role of Redox Stress and Band 3 Modifications [J].
Arese, Paolo ;
Gallo, Valentina ;
Pantaleo, Antonella ;
Turrini, Franco .
TRANSFUSION MEDICINE AND HEMOTHERAPY, 2012, 39 (05) :328-334
[5]  
Baker M A, 1984, Toxicol Pathol, V12, P331
[6]   Glucose-6-phosphate dehydrogenase deficiency and antimalarial drug development [J].
Beutler, Ernest ;
Duparc, Stephan .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2007, 77 (04) :779-789
[7]   PRESENCE IN VICIA-FABA OF DIFFERENT SUBSTANCES WITH ACTIVITY IN-VITRO ON GD(-)MED RED BLOOD CELL REDUCED GLUTATHIONE [J].
BOTTINI, E ;
LUCARELLI, P ;
SPENNATI, GF ;
BUSINCO, L ;
PALMARINO, R .
CLINICA CHIMICA ACTA, 1970, 30 (03) :831-+
[8]   Nutritional value of faba bean (Vicia faba L.) seeds for feed and food [J].
Crepon, Katell ;
Marget, Pascal ;
Peyronnet, Corinne ;
Carrouee, Benoit ;
Arese, Paolo ;
Duc, Gerard .
FIELD CROPS RESEARCH, 2010, 115 (03) :329-339
[9]   EFFECT OF FACTORS OF FAVISM ON THE PROTEIN AND LIPID COMPONENTS OF RAT ERYTHROCYTE-MEMBRANE [J].
DAQUINO, M ;
GAETANI, S ;
SPADONI, MA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 731 (02) :161-167
[10]   ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].
FAIRBANKS, G ;
STECK, TL ;
WALLACH, DFH .
BIOCHEMISTRY, 1971, 10 (13) :2606-+