Influence of Melaleuca alternifolia oil nanoparticles on aspects of Pseudomonas aeruginosa biofilm

被引：44
作者：
Comin, Vanessa M. [1 ]
Lopes, Leonardo Q. S. [1 ,2 ]
Quatrin, Priscilla M. [1 ,2 ]
de Souza, Marcia E. [1 ,2 ]
Bonez, Pauline C. [1 ]
Pintos, Francieli G. [1 ]
Raffin, Renata P. [2 ]
Vaucher, Rodrigo de A. [1 ,2 ]
Martinez, Diego S. T. [3 ]
Santos, Roberto C. V. [1 ,2 ,4 ]
机构：
[1] Ctr Univ Franciscano, Lab Microbiol Res, BR-97010032 Santa Maria, RS, Brazil
[2] Ctr Univ Franciscano, Postgrad Program Nanosci, BR-97010032 Santa Maria, RS, Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13084970 Campinas, SP, Brazil
[4] Univ Fed Santa Maria, Hlth Sci Ctr, Dept Microbiol & Parasitol, Microbiol Lab, Santa Maria, RS, Brazil
来源：
MICROBIAL PATHOGENESIS | 2016年 / 93卷
关键词：
Melaleuca alternifolia; Nanoparticles; Pseudomonas aeruginosa PAO1; Biofilm; TEA TREE OIL; PHYSICOCHEMICAL CHARACTERIZATION; ESCHERICHIA-COLI; CANDIDA-ALBICANS; EPITHELIAL-CELLS; MOTILITY; ANTIBIOTICS; ADHESION; HYDROPHOBICITY; NANOCAPSULES;
D O I：
10.1016/j.micpath.2016.01.019
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The Pseudomonas aeruginosa is a gram-negative bacillus and frequent cause of infection. This microorganism is resistant intrinsically to various drugs. The P. aeruginosa is associated with the biofilm formation, which causes worsen the prognosis and difficulty the treatment. The influence of Melaleuca alternifolia oil or "tree of tee" oil (TTO) and TTO nanoparticles on adhesion of P. aeruginosa in buccal epithelial cells was investigated. Also was determined the antimicrobial and antibiofilm activity against this microorganism. The TTO nanoparticles were produced by deposition of preformed polymer and the physic-chemical properties of nanoparticles were measured by electrophoresis and dynamic light scattering. The characterization of nanoparticle showed acceptable values for diameter and zeta potential. The evaluation of antimicrobial and antibiofilm activity against P. aeruginosa PAO1 was performed by microdilution indicating the minimal inhibitory concentration, and the potential antibiofilm. It was verified the action on virulence factors such the motility, besides the influence on adhesion in buccal epithelial cells. Both oil and nanoparticles showed a decrease in adhesion of microorganisms to buccal cells, decrease of biofilm and interfering on P. aeruginosa PAO1 motility. The nanostructuration of TTO, shows be a viable alternative against formed biofilm microorganisms. (C) 2016 Elsevier Ltd. All rights reserved.
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页码：120 / 125
页数：6
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