Protective effects of Forsythia suspensa extract against oxidative stress induced by diquat in rats

被引：128

作者：

Lu, T. ^{[1
]}

Piao, X. L. ^{[2
]}

Zhang, Q. ^{[1
]}

Wang, D. ^{[1
]}

Piao, X. S. ^{[1
]}

Kim, S. W. ^{[3
]}

机构：

[1] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

[2] Minzu Univ China, Inst Chinese Minor Tradit Med, Beijing 100081, Peoples R China

[3] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2010年 / 48卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Diquat; Forsythia suspensa extract; Oxidative stress; Rat; OXIDANT STRESS; ANTIOXIDANT; GLUTATHIONE; LIGNANS; PERFORMANCE; MECHANISMS; ASSAY;

D O I：

10.1016/j.fct.2009.12.018

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Forsythia suspensa extract has been proved as a potential antioxidant in the recent years. The present study was undertaken to obtain the optimal antioxidant fraction in vitro and examine its antioxidative potential against diquat-induced oxidative stress in male Sprague Dawley rats in vivo. In vitro, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging experiment indicated that the CH2Cl2 fraction of F suspensa (FSC) exerted the strongest scavenging activities; forsythoside A, forythialan A and phillygenin from it might be the major antioxiclant constituents. In vivo; pretreatment of rats with different doses of FSC (25, 50 and 100 mg/kg bw) and vitamin C (100 mg/kg bw, positive control) for 15 days significantly lowered the tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) in plasma compared to the negative control group. Also, FSC significantly increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and the levels of glutathione (GSH) in plasma, liver and kidney whereas it decreased the levels of malondialdehyde (MDA) in plasma and kidney. Moreover, the protective effect of FSC (100 mg/kg bw) was better than vitamin C. These results revealed that FSC exerted a protective effect against diquat-induced oxidative stress and is worthy of becoming a potential dietary antioxidant. (C) 2009 Elsevier Ltd. All rights reserved

引用

页码：764 / 770

页数：7

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