Thiourea mediated regulation in the expression profile of aquaporins and its impact on water homeostasis under salinity stress in Brassica juncea roots

被引：30

作者：

Srivastava, A. K. ^{[1
]}

Suprasanna, P. ^{[1
]}

Srivastava, S. ^{[2
]}

D'Souza, S. F. ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India

[2] Tata Inst Fundamental Res, Natl Facil High Field NMR, Bombay 400005, Maharashtra, India

来源：

PLANT SCIENCE | 2010年 / 178卷 / 06期

关键词：

Aquaporin; ROS; Real-time PCR; Salinity stress; Thiourea; Water homeostasis; PLASMA-MEMBRANE AQUAPORINS; ABIOTIC STRESSES; SALT STRESS; ARABIDOPSIS; PLANTS; TOBACCO; TOLERANCE; BARLEY; RICE;

D O I：

10.1016/j.plantsci.2010.02.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioregulatory molecules such as thiourea (TU) play an important role in imparting stress tolerance to crops. However, the molecular mechanism involved in the TU-mediated tolerance has not been elucidated. Towards this endeavour, the expression profile of various PIPs (plasma membrane intrinsic proteins) was studied under salt stress (NaCl; 700 mM) with/without thiourea (TU; 6.5 mM) at different time periods in roots of Brassica juncea. Various aquaporin isoforms demonstrated an upregulation upon salinity stress imposition, whereas they were downregulated upon TU supplementation. TU treatment also led to a decrease in the accumulation of reactive oxygen species and delimited the need for an enhanced accumulation of osmolytes. The vacuolar pH was also maintained in NaCl +TU treatment as demonstrated by in vivo P-31 NMR of roots. In conclusion, TU supplementation to salt stressed seedlings was found to maintain the water homeostasis of roots through coordinated regulation of different PIP isoforms. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：517 / 522

页数：6