Characterization and comparison of post-natal rat Achilles tendon-derived stem cells at different development stages

被引：34

作者：

Chen, Jialin ^{[1
,2
,4
]}

Zhang, Wei ^{[1
,2
]}

Liu, Zeyu ^{[1
,2
]}

Zhu, Ting ^{[1
,2
,3
]}

Shen, Weiliang ^{[1
,2
,3
]}

Ran, Jisheng ^{[3
]}

Tang, Qiaomei ^{[1
,2
,3
]}

Gong, Xiaonan ^{[1
,2
]}

Backman, Ludvig J. ^{[4
]}

Chen, Xiao ^{[1
,2
]}

Chen, Xiaowen ^{[5
,7
]}

Wen, Feiqiu ^{[5
]}

Ouyang, Hongwei ^{[1
,2
,6
,7
]}

机构：

[1] Zhejiang Univ, Sch Med, Ctr Stem Cell & Tissue Engn, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Prov Key Lab Tissue Engn & Regenerat Med, Hangzhou 310000, Zhejiang, Peoples R China

[3] Zhejiang Univ, Affiliated Hosp 2, Dept Orthopaed, Hangzhou 310009, Zhejiang, Peoples R China

[4] Umea Univ, Dept Integrat Med Biol, Anat, S-90187 Umea, Sweden

[5] Shenzhen Childrens Hosp, Div Haematol & Oncol, Shenzhen 518038, Peoples R China

[6] Zhejiang Univ, Sch Med, Dept Sports Med, Hangzhou 310058, Zhejiang, Peoples R China

[7] China Orthoped Regenerat Med Grp CORMed, Hangzhou 310058, Zhejiang, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

对外科技合作项目（国际科技项目）; 国家高技术研究发展计划(863计划);

关键词：

MARROW STROMAL CELLS; AGE-RELATED-CHANGES; STEM/PROGENITOR CELLS; SILK SCAFFOLD; FETAL; BONE; EXPRESSION; EFFICACY; REPAIR; MSCS;

D O I：

10.1038/srep22946

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tendon stem/progenitor cells (TSPCs) are a potential cell source for tendon tissue engineering. The striking morphological and structural changes of tendon tissue during development indicate the complexity of TSPCs at different stages. This study aims to characterize and compare post-natal rat Achilles tendon tissue and TSPCs at different stages of development. The tendon tissue showed distinct differences during development: the tissue structure became denser and more regular, the nuclei became spindle-shaped and the cell number decreased with time. TSPCs derived from 7 day Achilles tendon tissue showed the highest self-renewal ability, cell proliferation, and differentiation potential towards mesenchymal lineage, compared to TSPCs derived from 1 day and 56 day tissue. Microarray data showed up-regulation of several groups of genes in TSPCs derived from 7 day Achilles tendon tissue, which may account for the unique cell characteristics during this specific stage of development. Our results indicate that TSPCs derived from 7 day Achilles tendon tissue is a superior cell source as compared to TSPCs derived from 1 day and 56 day tissue, demonstrating the importance of choosing a suitable stem cell source for effective tendon tissue engineering and regeneration.

引用

页数：11

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