Identification of a FasL-like molecule in leucocytes of the teleost fish gilthead seabream (Sparus aurata L.)

被引：27
|
作者：
Cuesta, A [1 ]
Esteban, MA [1 ]
Meseguer, J [1 ]
机构：
[1] Univ Murcia, Dept Cell Biol, Fac Biol, E-30100 Murcia, Spain
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2003年 / 27卷 / 01期
关键词：
FasL; leucocytes; natural cytotoxic cells; immunoblotting; immunocytochemistry; teleosts; gilthead seabream (Sparus aurata L.);
D O I：
10.1016/S0145-305X(02)00041-1
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The possible expression of FasL in gilthead seabream leucocytes was studied by flow cytometry, immunoblotting and immunocytochemistry, using an anti-mouse FasL monoclonal antibody. The results pointed to a cytosolic FasL-like, but not a membrane-like form, in resting leucocytes from head-kidney, thymus, spleen, blood and peritoneal exudate. Immunoblotting revealed a 19 kDa. band in resting leucocytes, while activated leucocytes showed the same band and another of 39 kDa. The FasL-like molecule is identified in lymphocytes, monocyte-macrophages and acidophilic granulocytes. Phylogenetical and functional implications are suggested. (C) 2002 Elsevier Science Ltd. All. rights reserved.
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收藏
页码：21 / 27
页数：7
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