Identification of a FasL-like molecule in leucocytes of the teleost fish gilthead seabream (Sparus aurata L.)

被引：27

作者：

Cuesta, A ^{[1
]}

Esteban, MA ^{[1
]}

Meseguer, J ^{[1
]}

机构：

[1] Univ Murcia, Dept Cell Biol, Fac Biol, E-30100 Murcia, Spain

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2003年 / 27卷 / 01期

关键词：

FasL; leucocytes; natural cytotoxic cells; immunoblotting; immunocytochemistry; teleosts; gilthead seabream (Sparus aurata L.);

D O I：

10.1016/S0145-305X(02)00041-1

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The possible expression of FasL in gilthead seabream leucocytes was studied by flow cytometry, immunoblotting and immunocytochemistry, using an anti-mouse FasL monoclonal antibody. The results pointed to a cytosolic FasL-like, but not a membrane-like form, in resting leucocytes from head-kidney, thymus, spleen, blood and peritoneal exudate. Immunoblotting revealed a 19 kDa. band in resting leucocytes, while activated leucocytes showed the same band and another of 39 kDa. The FasL-like molecule is identified in lymphocytes, monocyte-macrophages and acidophilic granulocytes. Phylogenetical and functional implications are suggested. (C) 2002 Elsevier Science Ltd. All. rights reserved.

引用

页码：21 / 27

页数：7

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