Dihydroflavonol 4-reductase activity in lignocellulosic tissues

被引：17

作者：

Dellus, V

Heller, W

Sadermann, H

Scalbert, A

机构：

[1] INRA,CHIM BIOL LAB,F-78850 THIVERVAL GRIGNON,FRANCE

[2] GSF,INST BIOCHEM PFLANZENPATHOL,D-85764 OBERSCHLEISSHEIM,GERMANY

来源：

PHYTOCHEMISTRY | 1997年 / 45卷 / 07期

关键词：

Picea abies; Pinus sylvestris; Larix decidua; Pinaceae; dihydroflavonol; 4-reductase; extraction; assay; bark; sapwood; conifers;

D O I：

10.1016/S0031-9422(97)00128-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two enzyme assays using either radiolabelled or non-labelled substrates were applied to detect dihydroflavonol 4-reductase (DFR) activity in lignocellulosic tissues. The successful detection of DFR in these tissues rich in polyphenols relied on the addition of sufficient amounts of polyethylene glycol. DFR activity is measured in sapwood and inner bark of different conifer species. (C) 1997 Elsevier Science Ltd. All rights reserved.

引用

页码：1415 / 1418

页数：4

共 15 条

[1] EXTRACTION OF ENZYMES AND SUBCELLULAR ORGANELLES FROM PLANT TISSUES [J].