共 22 条
Effects of (-)-epigallocatechin-3-gallate on the activity of substantia nigra dopaminergic neurons
被引:11
作者:
Jeong, Han-Seong
Jang, Sujeong
Jang, Myung-Joo
Lee, Sam-Gyu
Kim, Tae-Sun
Tag-Heo
Lee, Jae-Hyuk
Jun, Jae Yeoul
Park, Jong-Seong
[1
]
机构:
[1] Chonnam Natl Univ, Sch Med, Dept Physiol, Kwangju 501190, South Korea
[2] Chonnam Natl Univ, Sch Med, Dept Phys Med & Rehabil, Kwangju 501190, South Korea
[3] Chonnam Natl Univ, Sch Med, Dept Neurosurg, Kwangju 501190, South Korea
[4] Chonnam Natl Univ, Sch Med, Dept Emergency Med, Kwangju 501190, South Korea
[5] Chonnam Natl Univ, Sch Med, Dept Pathol, Kwangju 501190, South Korea
[6] Chosun Univ, Coll Med, Dept Physiol, Kwangju 375, South Korea
关键词:
EGCG;
action potential;
substantia nigra;
dopaminergic neuron;
D O I:
10.1016/j.brainres.2006.10.078
中图分类号:
Q189 [神经科学];
学科分类号:
071006 ;
摘要:
Despite many studies on the biological and pharmacological properties of (-)-epigallocatechin-3-gallate (EGCG), an active component of green tea, information on neuronal modulation by EGCG is limited. This study was designed to investigate the effects of EGCG on the electrical activity of rat substantia nigra dopaminergic neurons using whole-cell patch clamp recordings. The spike frequency was increased to 6.33 +/- 0.23 (p < 0.05) and 7.15 +/- 0.29 (p < 0.05) by 5 and 10 mu M EGCG, respectively, from the control level of 5.49 +/- 0.19 spikes/second, respectively (n=18). The resting membrane potential of the cells was decreased to -45.66 +/- 0.45 and -43.99 +/- 0.87 (p < 0.05), by 5 and 10 mu M EGCG, respectively, from -47.82 +/- 0.57 mV. The amplitude of afterhyperpolarization was decreased to 12.73 +/- 0.45 (p < 0.05) and 11.60 +/- 0.57 (p < 0.05) by 5 and 10 mu M EGCG, respectively, from 13.80 +/- 0.31 mV. The neuronal activity of dopaminergic neurons is closely linked to dopamine release. When neurons switch from a single-spike firing to bursts of action potentials, the release of dopamine increases. The above experimental results suggest that EGCG increases the neuronal activity via inhibition of calcium-dependent potassium currents underlying the afterhyperpolarization, and it could act as a facilitating factor that elicits NMDA-dependent bursts of action potentials like apamin or bicuculline methiodide. (c) 2006 Elsevier B.V. All rights reserved.
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页码:114 / 118
页数:5
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