Bioinformatics analyses of the differences between lung adenocarcinoma and squamous cell carcinoma using The Cancer Genome Atlas expression data

被引:22
作者
Sun, Fenghao [1 ]
Yang, Xiaodong [1 ]
Jin, Yulin [1 ]
Chen, Li [1 ]
Wang, Lin [1 ]
Shi, Mengkun [1 ]
Zhan, Cheng [1 ]
Shi, Yu [1 ]
Wang, Qun [1 ]
机构
[1] Fudan Univ, Zhongshan Hosp, Dept Thorac Surg, 180 Fenglin Rd, Shanghai 200032, Peoples R China
基金
中国国家自然科学基金; 上海市自然科学基金;
关键词
lung cancer; differentially expressed genes; differentially expressed microRNAs; Gene Ontology analysis; Kyoto Encyclopedia of Genes and Genomes pathway analysis; transcription factor-miRNA-gene network; ACTIVATED RECEPTOR-GAMMA; PATHOLOGICAL DIAGNOSIS; DOWN-REGULATION; SMALL BIOPSIES; MIR-375; MICRORNA-1/133A; CLASSIFICATION; MANAGEMENT; BIOMARKER; TARGET;
D O I
10.3892/mmr.2017.6629
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The present study aimed to explore gene and microRNA (miRNA) expression differences between lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were identified by analyzing mRNA and miRNA expression data in normal and cancerous lung tissues that were obtained from The Cancer Genome Atlas database. A total of 778 DEGs and 7 DEMs were identified. Altered gene functions and signaling pathways were investigated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, which revealed that DEGs were significantly enriched in extracellular matrix organization, cell differentiation, negative regulation of toll signaling pathway, and several other terms and pathways. Transcription factor (TF)-miRNA-gene networks in LUAD and LUSC were predicted using the TargetScan, Miranda, and TRANSFAC databases, which revealed the regulatory links among the TFs, DEMs, and DEGs. The central TFs, i.e., the TFs in the middle of the TF-miRNA-gene network, of LUAD and LUSC were similar. Although LUAD and LUSC shared similar miRNAs in the predicted networks, miR-29b-3p was demonstrated to be upregulated only in LUAD, whereas miR-1, miR-105-5p, and miR-193b-5p were altered in LUSC. These findings may improve our understanding of the different molecular mechanisms in non-small cell lung cancers and may promote new and accurate strategies for prevention, diagnosis, and treatment.
引用
收藏
页码:609 / 616
页数:8
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