Unsuitability of Quantitative Bacteroidales 16S rRNA Gene Assays for Discerning Fecal Contamination of Drinking Water

被引：27

作者：

van der Wielen, Paul W. J. J. ^{[1
]}

Medema, Gertjan ^{[1
]}

机构：

[1] KWR Watercycle Res Inst, NL-3430 BB Nieuwegein, Netherlands

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 14期

关键词：

RICE-FIELD SOIL; FRAGILIS GROUP ORGANISMS; TIME PCR ASSAYS; SP-NOV; PLANT RESIDUE; DISTRIBUTION-SYSTEMS; INDICATOR BACTERIA; BACTERIOPHAGES MS2; ESCHERICHIA-COLI; FRESH-WATER;

D O I：

10.1128/AEM.03090-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacteroidales species were detected in (tap) water samples from treatment plants with three different PCR assays. 16S rRNA gene sequence analysis indicated that the sequences had an environmental rather than fecal origin. We conclude that assays for Bacteroidales 16S rRNA genes are not specific enough to discern fecal contamination of drinking water in the Netherlands.

引用

页码：4876 / 4881

页数：6

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