A general method for gene isolation in tagging approaches: amplification of insertion mutagenised sites (AIMS)

被引：50

作者：

Frey, M

Stettner, C

Gierl, A

机构：

[1] Tech Univ Munich, Genet Inst, D-85747 Garching, Germany

[2] Tech Univ Munich, Lehrstuhl Genet, D-85747 Garching, Germany

来源：

PLANT JOURNAL | 1998年 / 13卷 / 05期

关键词：

D O I：

10.1046/j.1365-313X.1998.00091.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.

引用

页码：717 / 721

页数：5

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