Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

被引：54

作者：

Fitzgerald, Collette ^{[1
]}

Tu, Zheng Chao ^{[2
]}

Patrick, Mary ^{[1
]}

Stiles, Tracy ^{[3
]}

Lawson, Andy J. ^{[4
]}

Santovenia, Monica ^{[1
]}

Gilbert, Maarten J. ^{[5
,6
]}

van Bergen, Marcel ^{[6
,7
]}

Joyce, Kevin ^{[1
]}

Pruckler, Janet ^{[1
]}

Stroika, Steven ^{[1
]}

Duim, Birgitta ^{[5
,6
]}

Miller, William G. ^{[8
]}

Loparev, Vladimir ^{[1
]}

Sinnige, Jan C. ^{[9
]}

Fields, Patricia I. ^{[1
]}

Tauxe, Robert V. ^{[1
]}

Blaser, Martin J. ^{[2
]}

Wagenaar, Jaap A. ^{[5
,6
,7
]}

机构：

[1] CDC, Biotechnol Core Facil Branch, Div Sci Resources, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA

[2] NYU, Dept Med, Sch Med, New York, NY 10016 USA

[3] Massachusetts Dept Publ Hlth, Jamaica Plain, MA USA

[4] Publ Hlth England, London, England

[5] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Utrecht, Netherlands

[6] WHO Collaborating Ctr Campylobacter, OIE Reference Lab Campylobacteriosis, Utrecht, Netherlands

[7] Cent Vet Inst Wageningen UR, Lelystad, Netherlands

[8] ARS, USDA, WRRC, Prod Safety & Microbiol Res Unit, Albany, CA USA

[9] Univ Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2014年 / 64卷

关键词：

GENUS CAMPYLOBACTER; PCR ASSAY; DIFFERENTIATION; IDENTIFICATION; STRAINS;

D O I：

10.1099/ijs.0.057778-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n=8) and reptiles (n=5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) (=ATCC BAA-2539(T)=LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

引用

页码：2944 / 2948

页数：5

共 22 条

[1] DIFFERENTIATION OF CAMPYLOBACTER SPECIES USING PHENOTYPIC CHARACTERIZATION [J].

BARRETT, TJ ;

PATTON, CM ;

MORRIS, GK .

LABORATORY MEDICINE, 1988, 19 (02) :96-102

[2] ESCHERICHIA-VULNERIS - A NEW SPECIES OF ENTEROBACTERIACEAE ASSOCIATED WITH HUMAN WOUNDS [J].

BRENNER, DJ ;

MCWHORTER, AC ;

KNUTSON, JKL ;

STEIGERWALT, AG .

JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (06) :1133-1140

[3] Genetic Relationships among Reptilian and Mammalian Campylobacter fetus Strains Determined by Multilocus Sequence Typing [J].

Dingle, Kate E. ;

Blaser, Martin J. ;

Tu, Zheng-Chao ;

Pruckler, Janet ;

Fitzgerald, Collette ;

van Bergen, Marcel A. P. ;

Lawson, Andrew J. ;

Owen, Robert J. ;

Wagenaar, Jaap A. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (03) :977-980

[4] Complete Genome Sequence of Campylobacter fetus subsp. testudinum Strain 03-427(T) [J].

Gilbert, Maarten J. ;

Miller, William G. ;

Yee, Emma ;

Blaser, Martin J. ;

Wagenaar, Jaap A. ;

Duim, Birgitta .

GENOME ANNOUNCEMENTS, 2013, 1 (06)

[5] ISOLATION OF CAMPYLOBACTER-FETUS FROM A PET TURTLE [J].

HARVEY, S ;

GREENWOOD, JR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (02) :260-261

[6] Evaluation of a PCR assay for identification and differentiation of Campylobacter fetus subspecies [J].

Hum, S ;

Quinn, K ;

Brunner, J ;

On, SLW .

AUSTRALIAN VETERINARY JOURNAL, 1997, 75 (11) :827-831

[7] Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals [J].

Linton, D ;

Owen, RJ ;

Stanley, J .

RESEARCH IN MICROBIOLOGY, 1996, 147 (09) :707-718

[8] Cell type-specific localization of sphingosine kinase 1a in human tissues [J].

Murate, T ;

Banno, Y ;

T-Koizumi, K ;

Watanabe, K ;

Mori, N ;

Wada, A ;

Igarashi, Y ;

Takagi, A ;

Kojima, T ;

Asano, H ;

Akao, Y ;

Yoshida, S ;

Saito, H ;

Nozawa, Y .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2001, 49 (07) :845-855

[9] Human Infections with New Subspecies of Campylobacter fetus [J].

Patrick, Mary E. ;

Gilbert, Maarten J. ;

Blaser, Martin J. ;

Tauxe, Robert V. ;

Wagenaar, Jaap A. ;

Fitzgerald, Collette .

EMERGING INFECTIOUS DISEASES, 2013, 19 (10) :1678-1680

[10] Rapid pulsed-field gel electrophoresis protocol for subtyping of Campylobacter jejuni [J].

Ribot, EM ;

Fitzgerald, C ;

Kubota, K ;

Swaminathan, B ;

Barrett, TJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) :1889-1894

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