Sequence analysis of the gene encoding H antigen in Escherichia coli isolated from food in Morocco

被引：2

作者：

Badri, Samira ^{[1
]}

Fassouane, Aziz ^{[2
]}

Filliol, Ingrid ^{[3
]}

Hassar, Mohammed ^{[1
]}

Cohen, Nozha ^{[1
]}

机构：

[1] Inst Pasteur Maroc, Lab Microbiol & Hyg Aliments & Environm, Casablanca 20100, Morocco

[2] Univ Chouaib, Fac Sci Eljadida, Dept Biol, Biochim Lab, Doukkali 24000, Morocco

[3] Inst Pasteur, Unit Biodivers Bacteries Pathogenes Emergentes, Ctr Natl Reference Escherichia Coli & Shigella, F-75015 Paris, France

来源：

JOURNAL OF MICROBIOLOGY | 2010年 / 48卷 / 02期

关键词：

E; coli; H-antigen; fliC gene; sequence analysis; PCR-RFLP; AMPLIFIED FLIC GENE; O26; STRAINS; RESTRICTION; IDENTIFICATION; VIRULENCE;

D O I：

10.1007/s12275-010-9182-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In order to develop other molecular method useful for typing of motile and non motile Escherichia coli strains, a total of 207 strains of E. coli (133 reference strains, 74 food strains) were characterized by analysis of sequences of their amplified flagellin-encoding (fliC) gene products. The collection of reference strains was used for database building of fliC gene sequences. Application of this identification system to 74 E. coli food isolates revealed a reproducible and clear cut classification with very good correlation to results obtained by HhaI restriction of the amplified flagellin gene. The proposed determination of fliC sequences variations should be helpful for epidemiological studies.

引用

页码：184 / 187

页数：4

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