Amblyomma sculptum tick saliva: α-Gal identification, antibody response and possible association with red meat allergy in Brazil

被引：86

作者：

Araujo, Ricardo Nascimento ^{[1
]}

Franco, Paula Ferreira ^{[1
]}

Rodrigues, Henrique ^{[1
]}

Santos, Luiza C. B. ^{[1
]}

McKay, Craig S. ^{[2
]}

Sanhueza, Carlos A. ^{[2
]}

Nascimento Brito, Carlos Ramon ^{[1
]}

Azevedo, Maira Araujo ^{[1
]}

Venuto, Ana Paula ^{[1
]}

Cowan, Peter J. ^{[3
,4
]}

Almeida, Igor C. ^{[5
]}

Finn, M. G. ^{[2
]}

Marques, Alexandre F. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG, Brazil

[2] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

[3] St Vincents Hosp, Immunol Res Ctr, Melbourne, Vic 3065, Australia

[4] Univ Melbourne, Dept Med, Parkville, Vic 3052, Australia

[5] Univ Texas El Paso, Dept Biol Sci, Border Biomed Res Ctr, El Paso, TX 79912 USA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2016年 / 46卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Amblyomma sculptum; Alpha-Gal; Tick saliva; IgE; Red meat allergy; AUSTRALIAN PARALYSIS TICK; VIRUS-LIKE PARTICLES; INCREASED IMMUNOGENICITY; CAJENNENSE FABRICIUS; IXODES-HOLOCYCLUS; FOOD ALLERGY; EPITOPES; IXODIDAE; XENOTRANSPLANTATION; IGE;

D O I：

10.1016/j.ijpara.2015.12.005

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The anaphylaxis response is frequently associated with food allergies, representing a significant public health hazard. Recently, exposure to tick bites and production of specific IgE against alpha-galactosyl (alpha-Gal)-containing epitopes has been correlated to red meat allergy. However, this association and the source of terminal, non-reducing alpha-Gal-containing epitopes have not previously been established in Brazil. Here, we employed the alpha-1,3-galactosyltransferase knockout mouse (alpha 1,3-GalT-KO) model and bacteriophage Q beta-virus like particles (Q beta-VLPs) displaying Gal alpha 1,3Gal beta 1,4GlcNAc (Gal alpha 3LN) epitopes to investigate the presence of alpha-Gal-containing epitopes in the saliva of Amblyomma sculptum, a species of the Amblyomma cajennense complex, which represents the main tick that infests humans in Brazil. We confirmed that the alpha-1,3-galactosyltransferase knockout animals produce significant levels of anti-alpha-Gal antibodies against the Gal alpha 1,3Gal beta 1,4GlcNAc epitopes displayed on Q beta-virus like particles. The injection of A. sculptum saliva or exposure to feeding ticks was also found to induce both IgG and IgE anti-alpha-Gal antibodies in alpha-1,3-galactosyltransferase knockout mice, thus indicating the presence of alpha-Gal containing epitopes in the tick saliva. The presence of alpha-Gal-containing epitopes was confirmed by ELISA and immunoblotting following removal of terminal alpha-Gal epitopes by alpha-galactosidase treatment. These results suggest for the first known time that bites from the A. sculptum tick may be associated with the unknown etiology of allergic reactions to red meat in Brazil. (C) 2016 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：213 / 220

页数：8

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