Production and characterization of specific monoclonal antibodies of the human thyroid stimulating hormone

被引：2

作者：

Conde, IBB ^{[1
]}

Moreno, AIB ^{[1
]}

Sanchéz, IA ^{[1
]}

Bas, CA ^{[1
]}

机构：

[1] Ctr Inmunoensayo, Lab Anticuerpos Monoclonales, C Habana, Cuba

来源：

HYBRIDOMA | 2000年 / 19卷 / 04期

关键词：

D O I：

10.1089/027245700429891

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Spleen cells from BALB/c mice immunized with human thyroid stimulating hormone (beta-subunit) were fused with mouse myeloma cells (P3/X63-Ag8) and five hybridomas secreting monoclonal antibodies (MAbs) were obtained. These hybridomas specifically recognize (hTSH) and do not cross-react with the other human glycoprotein hormones such as: luteinizing hormone (LH), follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hcG). The MAbs were of the IgG(1) subclass and ascitic fluid from these hybridomas was purified by affinity chromatography on Protein A-sepharose CL-4B column to isolate the IgG(1) active fraction. The affinity constant of these MAbs ranged from 3.2 x 10(10) to 1.5 x 10(11) M-1.

引用

页码：335 / 338

页数：4

共 15 条

[1] MEASUREMENT OF MONOCLONAL-ANTIBODY AFFINITY BY NONCOMPETITIVE ENZYME-IMMUNOASSAY [J].

BEATTY, JD ;

BEATTY, BG ;

VLAHOS, WG .

JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 100 (1-2) :173-179

[2] MONOCLONAL-ANTIBODY MAPPING OF THE ANTIGENIC SURFACE OF HUMAN THYROTROPIN AND ITS SUBUNITS [J].

BENKIRANE, MM ;

BON, D ;

COSTAGLIOLA, S ;

PAOLUCCI, F ;

DARBOURET, B ;

PRINCE, P ;

CARAYON, P .

ENDOCRINOLOGY, 1987, 121 (03) :1171-1177

[3] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES DIRECTED AGAINST DIFFERENT EPITOPES OF HUMAN THYROID STIMULATING HORMONE [J].

BRAUN, JM ;

CHARREIRE, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 132 (02) :197-263

[4] PRODUCTION OF MONOCLONAL-ANTIBODIES - STRATEGY AND TACTICS [J].

DESTGROTH, SF ;

SCHEIDEGGER, D .

JOURNAL OF IMMUNOLOGICAL METHODS, 1980, 35 (1-2) :1-21

[5]

DUSSAULT JH, 1983, CONGENITAL HYPOTHYRO, P45

[6] EPITOPE MAPPING OF HUMAN THYROTROPIN [J].

ENDO, Y ;

MIYAI, K ;

IIJIMA, Y ;

NAKAJIMA, T ;

EDA, Y ;

FUJITA, H ;

UNOKI, M .

ACTA ENDOCRINOLOGICA, 1989, 120 (02) :201-209

[7] ANTIBODIES TO MAJOR HISTOCOMPATIBILITY ANTIGENS PRODUCED BY HYBRID CELL LINES [J].

GALFRE, G ;

HOWE, SC ;

MILSTEIN, C ;

BUTCHER, GW ;

HOWARD, JC .

NATURE, 1977, 266 (5602) :550-552

[8]

HAYASHIZAKI Y, 1985, FEBS LETT, V188, P394, DOI 10.1016/0014-5793(85)80409-9

[9] CHARACTERIZATION OF A BIOEFFECTIVE MONOCLONAL-ANTIBODY AGAINST THYROTROPIN BETA-SUBUNIT [J].

HILL, BL ;

LUSTBADER, JW ;

ERLANGER, BF .

ENDOCRINOLOGY, 1987, 120 (02) :574-581

[10] CRYSTAL-STRUCTURE OF HUMAN CHORIONIC-GONADOTROPIN [J].

LAPTHORN, AJ ;

HARRIS, DC ;

LITTLEJOHN, A ;

LUSTBADER, JW ;

CANFIELD, RE ;

MACHIN, KJ ;

MORGAN, FJ ;

ISAACS, NW .

NATURE, 1994, 369 (6480) :455-461

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