Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy

被引：30

作者：

Zeng, Weihong ^{[1
]}

Liu, Zhicui ^{[2
]}

Liu, Xinmei ^{[1
]}

Zhang, Siming ^{[1
]}

Khanniche, Asma ^{[3
]}

Zheng, Ying ^{[4
]}

Ma, Xiaoling ^{[1
]}

Yu, Tiantian ^{[1
]}

Tian, Fuju ^{[1
]}

Liu, Xiao-Rui ^{[1
]}

Fan, Jianxia ^{[1
]}

Lin, Yi ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Inst Embryofetal Original Adult Dis, Int Peace Matern & Child Hlth Hosp,Sch Med, Shanghai, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Dermatol, Shanghai, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai Inst Immunol, Shanghai, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Out Patient Operating Room, Shanghai, Peoples R China

来源：

FRONTIERS IN IMMUNOLOGY | 2017年 / 8卷

基金：

中国国家自然科学基金;

关键词：

decidual CD4(+) T cells; early human pregnancy; transcriptome; alternative splicing; high-throughput mRNA sequencing; MATERNAL-FETAL INTERFACE; RNA SEQUENCING REVEALS; EARLY-STAGE; NK CELLS; DIFFERENTIAL EXPRESSION; ACTIVATION STATUS; INTRON RETENTION; LYMPHOID-CELLS; CENTRAL MEMORY; LYMPHOCYTES;

D O I：

10.3389/fimmu.2017.00682

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Decidual CD4(+) T (dCD4 T) cells are crucial for the maternal-fetal immune tolerance required for a healthy pregnancy outcome. However, their molecular and functional characteristics are not well elucidated. In this study, we performed the first analysis of transcriptional and alternative splicing (AS) landscapes for paired decidual and peripheral blood CD4+ T (pCD4 T) cells in human early pregnancy using high throughput mRNA sequencing. Our data showed that dCD4 T cells are endowed with a unique transcriptional signature when compared to pCD4 T cells: dCD4 T cells upregulate 1,695 genes enriched in immune system process whereas downregulate 1,011 genes mainly related to mRNA catabolic process and the ribosome. Moreover, dCD4 T cells were observed to be at M phase, and show increased activation, proliferation, and cytokine production, as well as display an effector-memory phenotype and a heterogenous nature containing Th1, Th17, and Treg cell subsets. However, dCD4 T cells undergo a comparable number of upregulated and downregulated AS events, both of which are enriched in the genes related to cellular metabolic process. And the changes at the AS event level do not reflect measurable differences at the gene expression level in dCD4 T cells. Collectively, our findings provide a comprehensive portrait of the unique transcriptional signature and AS profile of CD4+ T cells in human decidua and help us gain more understanding of the functional characteristic of these cells during early pregnancy.

引用

页数：15

共 85 条

[1] Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells [J].