Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital

被引:48
作者
Winkelmann, Ria [1 ]
Schneider, Markus [1 ]
Hartmann, Sylvia [1 ]
Schnitzbauer, Andreas A. [2 ]
Zeuzem, Stefan [3 ]
Peveling-Oberhag, Jan [3 ,4 ]
Hansmann, Martin Leo [1 ]
Walter, Dirk [1 ,3 ]
机构
[1] Johann Wolfgang Goethe Univ Hosp, Senckenberg Inst Pathol, Theodor Stern Kai 7, D-60590 Frankfurt, Germany
[2] Johann Wolfgang Goethe Univ Hosp, Dept Gen & Visceral Surg, Theodor Stern Kai 7, D-60590 Frankfurt, Germany
[3] Johann Wolfgang Goethe Univ Hosp, Dept Internal Med 1, Theodor Stern Kai 7, D-60590 Frankfurt, Germany
[4] Robert Bosch Krankenhaus, Dept Gastroenterol Hepatol & Endocrinol, Auerbachstr 110, D-70376 Stuttgart, Germany
关键词
microsatellite instability; cholangiocarcinoma; immunohistochemistry; PCR; NONPOLYPOSIS COLORECTAL-CANCER; CELL LUNG-CANCER; NIVOLUMAB; IMMUNOHISTOCHEMISTRY; PCR;
D O I
10.3390/ijms19051421
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Immune-modulating therapy is a promising therapy for patients with cholangiocarcinoma (CCA). Microsatellite instability (MSI) might be a favorable predictor for treatment response, but comprehensive data on the prevalence of MSI in CCA are missing. The aim of the current study was to determine the prevalence of MSI in a German tertiary care hospital. Formalin-fixed paraffin-embedded tissue samples, obtained in the study period from 2007 to 2015 from patients with CCA undergoing surgical resection with curative intention at Johann Wolfgang Goethe University hospital, were examined. All samples were investigated immunohistochemically for the presence of MSI (expression of MLH1, PMS2, MSH2, and MSH6) as well as by pentaplex polymerase chain reaction for five quasimonomorphic mononucleotide repeats (BAT-25, BAT-26, NR-21, NR-22, and NR-24). In total, 102 patients were included, presenting intrahepatic (n = 35, 34.3%), perihilar (n = 42, 41.2%), and distal CCA (n = 25, 24.5%). In the immunohistochemical analysis, no loss of expression of DNA repair enzymes was observed. In the PCR-based analysis, one out of 102 patients was found to be MSI-high and one out of 102 was found to be MSI-low. Thus, MSI seems to appear rarely in CCA in Germany. This should be considered when planning immune-modulating therapy trials for patients with CCA.
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页数:10
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