Anti-inflammatory properties of phenolic compounds and crude extract from Porphyra dentata

被引:133
作者
Kazlowska, Katarzyna [1 ,2 ]
Hsu, Todd [3 ]
Hou, Chia-Chung [1 ]
Yang, Wen-Chin [1 ,4 ]
Tsai, Guo-Jane [2 ]
机构
[1] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan
[2] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung 20224, Taiwan
[3] Natl Taiwan Ocean Univ, Inst Biosci & Biotechnol, Chilung 20224, Taiwan
[4] Natl Taiwan Ocean Univ, Dept Aquaculture, Chilung 20224, Taiwan
关键词
iNOS; NO; NF-kappa B; Phenolics; Red alga; NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; ACID PHENETHYL ESTER; INTERFERON-GAMMA; IN-VITRO; GENE-EXPRESSION; LIPOPOLYSACCHARIDE; ACTIVATION; MACROPHAGES; PATHWAY;
D O I
10.1016/j.jep.2009.12.037
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological relevance: Porphyra dentata, a red edible seaweed, has long been used worldwide in folk medicine for the treatment of inflammatory diseases such as hypersensitivity, lymphadenitis, bronchitis. Aims of study: To clarify the anti-inflammatory role of Porphyra dentata crude extract and its identified phenolic compounds by investigating their effect on the nitric oxide (NO)/inducible nitric oxide synthase (iNOS) transcription pathway in macrophage RAW 264.7 cells. Materials and methods: Porphyra dentata crude extract was prepared with methanol. High performance liquid chromatography (HPLC) hyphenated to electrospray ionization mass spectrometry (ESI-MS) and UV detection were utilized to analyze the extract fingerprints. Nitrite measurement, iNOS promoter activity and nuclear factor-kappa B (NF-kappa B) enhancer activity were used to assess the anti-inflammatory effect in lipopolysaccharide (LPS) challenged mouse RAW 264.7 cell line. Results: Phenolic compounds (catechol, rutin and hesperidin) were identified in the crude extract of Porphyra dentata. The crude extract and the phenolic compounds inhibited the production of NO in LPS-stimulated RAW 264.7 cells. Catechol was a more potent suppressor of the up-regulation of iNOS promoter and NF-kappa B enhancer than rutin and yet, hesperidin alone failed to inhibit either activity. Conclusion: Our results indicate that catechol and rutin, but not hesperidin, are primary bioactive phenolic compounds in the crude extract to suppress NO production in LPS-stimulated macrophages via NF-kappa B-dependent iNOS gene transcription. The data also explain the anti-inflammatory use and possible mechanism of Porphyra dentata in iNOS implicated diseases. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:123 / 130
页数:8
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