Nerve growth factor treatment of sensory neuron primary cultures causes elevated levels of the mRNA encoding the ATP synthase beta-subunit as detected by a novel PCR-based differential cloning method

被引：4

作者：

Kendall, G ^{[1
]}

Ensor, E ^{[1
]}

Crankson, HD ^{[1
]}

Latchman, DS ^{[1
]}

机构：

[1] UCL, SCH MED, DEPT MOLEC PATHOL, MED MOLEC BIOL UNIT, LONDON W1P 6DB, ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1996年 / 236卷 / 02期

关键词：

gene regulation; sensory neurons; nerve growth factor; ATP synthase;

D O I：

10.1111/j.1432-1033.1996.00360.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mRNA encoding the rat ATP synthase beta-subunit was rapidly induced by nerve growth factor, within 60 min, in cultured adult rat dorsal root ganglion neurons. ATP synthase beta-subunit cDNA clones were isolated from a lambda library. The library was constructed using rat dorsal root ganglion mRNA that was differentially screened with cDNA-derived probes from untreated and nerve-growth-factor-treated primary cultures of adult rat dorsal root ganglion sensory neurons. Radiolabelled probes were made from submicrogram quantities of RNA, by a novel PCR-based technique, which allows small amounts of primary tissue to be used for library screening. The use of this technique in isolating novel differentially expressed mRNAs is discussed.

引用

页码：360 / 364

页数：5