A flow-through enzyme immunoassay for the screening of fumonisins in maize

被引：45
作者：
Paepens, C
De Saeger, S
Sibanda, L
Barna-Vetró, I
Léglise, I
Van Hove, F
Van Peteghem, C
机构：
[1] State Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium
[2] TOXI TEST NV, B-9000 Ghent, Belgium
[3] Inst Anim Sci, Ctr Agr Biotechnol, H-2101 Godollo, Hungary
[4] Univ Catholique Louvain, Nutr Biochem Unit, B-1348 Louvain, Belgium
[5] Univ Catholique Louvain, Mycotheque, BCCM MUCL, B-1348 Louvain, Belgium
来源：
ANALYTICA CHIMICA ACTA | 2004年 / 523卷 / 02期
关键词：
fumonisins; rapid screening method; HPLC;
D O I：
10.1016/j.aca.2004.07.044
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The format of an existing flow-through enzyme immunoassay has been optimised for the detection of fumonisin B-1 (FB1) and B-2 (FB2) in maize. The visual detection limit i.e. the smallest mycotoxin concentration resulting in no colour development, is 1000 mug/kg. Assay validation was performed using samples spiked with respectively only FBI and a FB1 + FB2 mixture (ratio 1:1). Results showed that within-day and between-day coefficients of variation ranged from respectively 0.4-10.2% and 1.1-9.0%. Naturally contaminated samples were screened with the developed flow-through method and results were compared with fumonisin contamination values obtained by a validated HPLC method. The assay was demonstrated to be accurate and reliable giving no false compliant and only a low percentage of false non-compliant results. The described method offers a simple, rapid and cost-effective screening tool, thus contributing to a better consumers' health protection. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：229 / 235
页数：7
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