A flow-through enzyme immunoassay for the screening of fumonisins in maize

被引：45

作者：

Paepens, C

De Saeger, S

Sibanda, L

Barna-Vetró, I

Léglise, I

Van Hove, F

Van Peteghem, C

机构：

[1] State Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium

[2] TOXI TEST NV, B-9000 Ghent, Belgium

[3] Inst Anim Sci, Ctr Agr Biotechnol, H-2101 Godollo, Hungary

[4] Univ Catholique Louvain, Nutr Biochem Unit, B-1348 Louvain, Belgium

[5] Univ Catholique Louvain, Mycotheque, BCCM MUCL, B-1348 Louvain, Belgium

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 523卷 / 02期

关键词：

fumonisins; rapid screening method; HPLC;

D O I：

10.1016/j.aca.2004.07.044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The format of an existing flow-through enzyme immunoassay has been optimised for the detection of fumonisin B-1 (FB1) and B-2 (FB2) in maize. The visual detection limit i.e. the smallest mycotoxin concentration resulting in no colour development, is 1000 mug/kg. Assay validation was performed using samples spiked with respectively only FBI and a FB1 + FB2 mixture (ratio 1:1). Results showed that within-day and between-day coefficients of variation ranged from respectively 0.4-10.2% and 1.1-9.0%. Naturally contaminated samples were screened with the developed flow-through method and results were compared with fumonisin contamination values obtained by a validated HPLC method. The assay was demonstrated to be accurate and reliable giving no false compliant and only a low percentage of false non-compliant results. The described method offers a simple, rapid and cost-effective screening tool, thus contributing to a better consumers' health protection. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：229 / 235

页数：7

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