Ultraviolet B irradiation-enhanced interleukin (IL)-6 production and mRNA expression are mediated by IL-1 alpha in cultured human keratinocytes

被引:78
作者
Chung, JH [1 ]
Youn, SH [1 ]
Koh, WS [1 ]
Eun, HC [1 ]
Cho, KH [1 ]
Park, KC [1 ]
Youn, JI [1 ]
机构
[1] SEOUL NATL UNIV,COLL MED,DEPT DERMATOL,SEOUL,SOUTH KOREA
关键词
cytokines;
D O I
10.1111/1523-1747.ep12345608
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Ultraviolet (UV) B radiation map trigger cutaneous inflammatory responses by directly inducing epidermal keratinocytes to elaborate specific cytokines such as interleukin (IL)-1 and IL-6. Because IL-1 is a potent inducer of IL-6, one may speculate that the release of IL-6 by keratinocytes after UV exposure is mediated via the release of IL-1 in an autocrine or paracrine manner. We demonstrated that UVB irradiation upregulated IL-1 alpha mRNA at a lower dose (15 mJ/cm(2)) and then downregulated IL-1 alpha mRNA expression at high doses (30-40 mJ/cm(2)). The kinetic profile of IL-1 alpha mRNA expression showed a biphasic response, with the early increase by 1 h after UV exposure and the secondary increase at 6 h after UV. On the other hand, the expression of IL-6 mRNA was increased with increasing doses of UVB (0-45 mJ/cm(2)) and showed a single peak at 6 h post-UV. These results may indicate that UVB radiation could regulate the expression of IL-1 alpha and IL-6 mRNA in keratinocytes by different mechanisms. Our data show that antihuman IL-1 alpha antibody inhibits UV-induced IL-6 production and mRNA expression in cultured keratinocytes. The addition of recombinant IL-1 alpha to the medium increased IL-6 synthesis and augmented IL-6 production and mRNA expression in cultured human keratinocytes by UVB irradiation, These results support the hypothesis that UVB irradiation-enhanced IL-6 production and mRNA expression may be mediated by IL-1 alpha.
引用
收藏
页码:715 / 720
页数:6
相关论文
共 27 条
[1]  
ANSEL J, 1990, J INVEST DERMATOL, V94, P101
[2]  
ANSEL JC, 1988, J IMMUNOL, V140, P2274
[3]  
Boyce S.T., 1983, J INVEST DERMATOL S, V81, P33
[4]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[5]   EFFECT OF ULTRAVIOLET-A ON IL-1 PRODUCTION BY ULTRAVIOLET-B IN CULTURED HUMAN KERATINOCYTES [J].
CHUNG, JH ;
YOUN, JI .
JOURNAL OF DERMATOLOGICAL SCIENCE, 1995, 9 (02) :87-93
[6]   POSTTRANSCRIPTIONAL REGULATION OF INTERLEUKIN-6 GENE-EXPRESSION IN HUMAN KERATINOCYTES BY ULTRAVIOLET-B RADIATION [J].
DEVOS, S ;
BRACH, M ;
BUDNIK, A ;
GREWE, M ;
HERRMANN, F ;
KRUTMANN, J .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 103 (01) :92-96
[7]   LOCALIZATION AND CHARACTERIZATION OF THE INTERLEUKIN-1 IMMUNOREACTIVE POOL (IL-1 ALPHA AND BETA FORMS) IN NORMAL HUMAN-EPIDERMIS [J].
DIDIERJEAN, L ;
SALOMON, D ;
MEROT, Y ;
SIEGENTHALER, G ;
SHAW, A ;
DAYER, JM ;
SAURAT, JH .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1989, 92 (06) :809-816
[8]   INTERLEUKIN-6 IN PSORIASIS - EXPRESSION AND MITOGENICITY STUDIES [J].
ELDER, JT ;
SARTOR, CI ;
BOMAN, DK ;
BENRAZAVI, S ;
FISHER, GJ ;
PITTELKOW, MR .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 1992, 284 (06) :324-332
[9]   REGULATION OF GM-CSF AND IL-3 PRODUCTION FROM THE MURINE KERATINOCYTE CELL-LINE PAM-212 FOLLOWING EXPOSURE TO ULTRAVIOLET-RADIATION [J].
GALLO, RL ;
STASZEWSKI, R ;
SAUDER, DN ;
KNISELY, TL ;
GRANSTEIN, RD .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (02) :203-209
[10]   DETERMINANTS AND REGULATION OF CYTOPLASMIC MESSENGER-RNA STABILITY IN EUKARYOTIC CELLS [J].
HENTZE, MW .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1090 (03) :281-292