Introduction of the archaebacterial geranylgeranyl pyrophosphate synthase gene into Chlamydomonas reinhardtii chloroplast

被引：16
|
作者：
Fukusaki, EI
Nishikawa, T
Kato, K
Shinmyo, A
Hemmi, H
Nishino, T
Kobayashi, A
机构：
[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan
[2] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan
[3] Tohoku Univ, Fac Engn, Dept Biochem & Engn, Aoba Ku, Sendai, Miyagi 9800866, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2003年 / 95卷 / 03期
关键词：
terpenoid; polyisoprene; prenyltransferase; Chlamydomonas; geranylgeranyldiphosphate synthase; chloroplast;
D O I：
10.1263/jbb.95.283
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Geranylgeranyl pyrophosphate (GGPP) synthase gene (gds) derived from a thermophilic Archae Sulfolobus acidocaldarius, was introduced into a unicellular green alga Chlamydomonas reinhardtii chloroplast. Heat treatment abolished the prenyltransferase activity of the wild strain, but the activity of the transformant remained. The transformant accumulated gds gene mRNA and translation product.
引用
收藏
页码：283 / 287
页数：5
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