Localized Translation of gurken/TGF-α mRNA during Axis Specification Is Controlled by Access to Orb/CPEB on Processing Bodies

被引：28

作者：

Davidson, Alexander ^{[1
]}

Parton, Richard M. ^{[1
]}

Rabouille, Catherine ^{[2
,3
,4
]}

Weil, Timothy T. ^{[5
]}

Davis, Ilan ^{[1
]}

机构：

[1] Univ Oxford, Dept Biochem, S Parks Rd, Oxford OX1 3QU, England

[2] Royal Netherlands Acad Arts & Sci, Hubrecht Inst, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

[3] Univ Med Ctr Utrecht, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

[4] UMC Utrecht, Dept Cell Biol, Heidelberglaan 100, NL-3584 CX Utrecht, Netherlands

[5] Univ Cambridge, Dept Zool, Downing St, Cambridge CB2 3EJ, England

来源：

CELL REPORTS | 2016年 / 14卷 / 10期

基金：

英国惠康基金;

关键词：

CYTOPLASMIC POLYADENYLATION; DROSOPHILA OOGENESIS; BINDING-PROTEIN; OSKAR TRANSLATION; NANOS TRANSLATION; OOCYTE MATURATION; GENE GURKEN; GERM PLASM; ORB; REPRESSION;

D O I：

10.1016/j.celrep.2016.02.038

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In Drosophila oocytes, gurken/TGF-alpha mRNA is essential for establishing the future embryonic axes. gurken remains translationally silent during transport from its point of synthesis in nurse cells to its final destination in the oocyte, where it associates with the edge of processing bodies. Here we show that, in nurse cells, gurken is kept translationally silent by the lack of sufficient Orb/CPEB, its translational activator. Processing bodies in nurse cells have a similar protein complement and ultrastructure to those in the oocyte, but they markedly less Orb and do not associate with gurken mRNA. Ectopic expression of Orb in nurse cells at levels similar to the wild-type oocyte dorso-anterior corner at mid-oogenesis is sufficient to cause gurken mRNA to associate with processing bodies and translate prematurely. We propose that controlling the spatial distribution of translational activators is a fundamental mechanism for regulating localized translation.

引用

页码：2451 / 2462

页数：12

共 71 条

[1] Localization of ASH1 mRNA particles in living yeast [J].

Bertrand, E ;

Chartrand, P ;

Schaefer, M ;

Shenoy, SM ;

Singer, RH ;

Long, RM .

MOLECULAR CELL, 1998, 2 (04) :437-445

[2] Drosophila PTB promotes formation of high-order RNP particles and represses oskar translation [J].

Besse, Florence ;

de Quinto, Sonia Lopez ;

Marchand, Virginie ;

Trucco, Alvar ;

Ephrussi, Anne .

GENES & DEVELOPMENT, 2009, 23 (02) :195-207

[3] Translational control of localized mRNAs: restricting protein synthesis in space and time [J].

Besse, Florence ;

Ephrussi, Anne .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (12) :971-980

[4] Drosophila Cornichon acts as cargo receptor for ER export of the TGFα-like growth factor Gurken [J].

Bökel, C ;

Dass, S ;

Wilsch-Bräuninger, M ;

Roth, S .

DEVELOPMENT, 2006, 133 (03) :459-470

[5] The Carnegie protein trap library: A versatile tool for Drosophila developmental studies [J].

Buszczak, Michael ;

Paterno, Shelley ;

Lighthouse, Daniel ;

Bachman, Julia ;

Planck, Jamie ;

Owen, Stephenie ;

Skora, Andrew D. ;

Nystul, Todd G. ;

Ohlstein, Benjamin ;

Allen, Anna ;

Wilhelm, James E. ;

Murphy, Terence D. ;

Levis, Robert W. ;

Matunis, Erika ;

Srivali, Nahathai ;

Hoskins, Roger A. ;

Spradling, Allan C. .

GENETICS, 2007, 175 (03) :1505-1531

[6] Translational repression of gurken mRNA in the Drosophila oocyte requires the hnRNP Squid in the nurse cells [J].

Caceres, Lucia ;

Nilson, Laura A. .

DEVELOPMENTAL BIOLOGY, 2009, 326 (02) :327-334

[7] Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation [J].

Cao, QP ;

Richter, JD .

EMBO JOURNAL, 2002, 21 (14) :3852-3862

[8] Orb and a long poly(A) tall are required for efficient oskar translation at the posterior pole of the Drosophila oocyte [J].

Castagnetti, S ;

Ephrussi, A .

DEVELOPMENT, 2003, 130 (05) :835-843

[9] The Drosophila CPEB homolog, Orb, is required for Oskar protein expression in oocytes [J].

Chang, JS ;

Tan, LH ;

Schedl, P .

DEVELOPMENTAL BIOLOGY, 1999, 215 (01) :91-106

[10] Bruno acts as a dual repressor of oskar translation, promoting mRNA oligomerization and formation of silencing particles [J].

Chekulaeva, M ;

Hentze, MW ;

Ephrussi, A .

CELL, 2006, 124 (03) :521-533

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