Human papillomavirus 16 E6 and E7 oncoprotein expression alters microRNA expression in extracellular vesicles

被引：60

作者：

Harden, Mallory E. ^{[1
,2
]}

Munger, Karl ^{[2
]}

机构：

[1] Harvard Med Sch, Div Med Sci, Program Virol, Boston, MA 02115 USA

[2] Tufts Univ, Sch Med, Dept Dev Mol & Chem Biol, 150 Harrison Ave,J607, Boston, MA 02111 USA

来源：

VIROLOGY | 2017年 / 508卷

关键词：

MicroRNA; Cervical cancer; Exosomes; Human papillomaviruses; APOPTOTIC BODIES; PROGENITOR CELLS; EXOSOMES; MICROVESICLES; COMMUNICATION; MEDIATORS; RECEPTOR; DISTINCT; BINDING;

D O I：

10.1016/j.virol.2017.05.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Extracellular vesicles released by cancer cells are mediators of intercellular communication that have been reported to contribute to carcinogenesis. Since they are readily detected in bodily fluids, they may also be used as cancer biomarkers. The E6/E7 oncoproteins drive human papillomavirus (HPV)-associated cancers, which account for approximately 5% of all human cancers worldwide. Here, we investigate how HPV16 E6/E7 oncogene expression in primary human epithelial cells alters miR expression in extracellular vesicles and compare these to changes in intracellular miR expression. Examining a panel of 68 cancer related miRs revealed that many miRs had similar expression patterns in cells and in extracellular vesicles, whereas some other miRs had different expression patterns and may be selectively packaged into extracellular vesicles. Interestingly, the set of miRs that may be selectively packaged in HPV16 E6/E7 extracellular vesicles is predicted to inhibit necrosis and apoptosis.

引用

页码：63 / 69

页数：7

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