Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution

被引：30

作者：

El-Shishtawy, Reda M. ^{[1
]}

Asiri, Abdullah M. ^{[1
]}

Basaif, Salem A. ^{[1
]}

Sobahi, T. Rashad ^{[1
]}

机构：

[1] King Abdulaziz Univ, Fac Sci, Dept Chem, Jeddah 21589, Saudi Arabia

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2010年 / 75卷 / 05期

关键词：

Monomethine cyanine dyes; Ethidium bromide; DNA; Napthothiazole; Fluorescence enhancement; Intercalation; DOUBLE-STRANDED DNA; FLUOROMETRIC ASSAY; NUCLEIC-ACIDS; RNA; PROBES; LIGHT; STATE;

D O I：

10.1016/j.saa.2010.02.026

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Novel monomethine cyanine dye (MC) derived from beta-naphthothiazole and benzothiazole has been prepared and characterized by H-1 and C-13 NMR, FTIR, ESIMS, elemental analyses, absorption and fluorescence spectroscopy. The dye was conveniently synthesized by the condensation of two sulfate heterocyclic quaternary salts. The interaction between calf thymus DNA (ct-DNA) in tris(hydroxymethyl)aminomethane-HCl (Tris-HCl) aqueous buffer solution and MC has been studied with spectral fluorescence method. The binding constant value has been determined by fluorescence titration of MC with ct-DNA concentrations. The result obtained is consistent with an intercalative binding interaction between MC and ct-DNA. Compared with ethidium bromide (EB). MC showed a huge fluorescence enhancement upon mixing with ct-DNA. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1605 / 1609

页数：5

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