Cyclin-Dependent Kinase 14 Promotes Cell Proliferation, Migration and Invasion in Ovarian Cancer by Inhibiting Wnt Signaling Pathway

被引：35

作者：

Jun Ou-Yang ^{[1
]}

Huang, Li-Hong ^{[1
]}

Sun, Xiang-Xiu ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Obstet & Gynecol, Tongji Med Coll, Affiliated Puai Hosp, 3rd Gutian Rd, Wuhan 430034, Hubei, Peoples R China

来源：

GYNECOLOGIC AND OBSTETRIC INVESTIGATION | 2017年 / 82卷 / 03期

关键词：

Ovarian cancer; Cyclin-dependent kinase 14; Wnt signaling pathway; SKOV3; beta-Catenin; Cell proliferation; Cell migration; Cell invasion; OVEREXPRESSION; CHEMOTHERAPY; CARCINOMA;

D O I：

10.1159/000447632

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: The study aimed to investigate cyclin-dependent kinase 14 (CDK14) and its co-function with Wnt signaling pathway on cell proliferation, migration and invasion in ovarian cancer. Methods: CDK14 expressions were detected by quantitative real-time polymerase chain reaction. The expressions c-Myc, cyclinD1, PFTK1, ki67 and OGT were examined by Western blot. MTT assay was applied to observe cell proliferation after transfection of pEGFP-N1/ CDK14-siRNA and pEGFP-N1 into SKOV3 cells, and scratch test and Transwell assay to observe invasion and migration ability. Transfected tumor model in nude mice was established. Results: CDK14 was upregulated in the ovarian cancer tissues and cell lines (both p < 0.05). Expressions of downstream molecules in Wnt signaling pathway as well as the proliferation, invasion and migration ability of the SKOV3 cells were reduced when CDK14 was inhibited (all p < 0.05). The expression of beta-catenin in the nucleus was also decreased when CDK14 was inhibited (p < 0.05). In the transfected tumor model of nude mice, the results showed, compared with the pEGFP-N1 group and blank control group, that the expressions of c-Myc, cyclinD1, PFTK1, ki67 and OGT in the pEGFP-N1/CDK14-siRNA group in the transplantation tumor tissues decreased significantly (all p < 0.05). Conclusion: CDK14 suppression-mediated Wnt signaling pathway can inhibit cell proliferation, invasion and migration in ovarian cancer. (C) 2016 S. Karger AG, Basel

引用

页码：230 / 239

页数：10

共 22 条

[1] Final overall survival and safety analysis of OCEANS, a phase 3 trial of chemotherapy with or without bevacizumab in patients with platinum-sensitive recurrent ovarian cancer [J].

Aghajanian, Carol ;

Goff, Barbara ;

Nycum, Lawrence R. ;

Wang, Yan V. ;

Husain, Amreen ;

Blank, Stephanie V. .

GYNECOLOGIC ONCOLOGY, 2015, 139 (01) :10-16

[2] Integrated genomic analyses of ovarian carcinoma [J].

Bell, D. ;

Berchuck, A. ;

Birrer, M. ;

Chien, J. ;

Cramer, D. W. ;

Dao, F. ;

Dhir, R. ;

DiSaia, P. ;

Gabra, H. ;

Glenn, P. ;

Godwin, A. K. ;

Gross, J. ;

Hartmann, L. ;

Huang, M. ;

Huntsman, D. G. ;

Iacocca, M. ;

Imielinski, M. ;

Kalloger, S. ;

Karlan, B. Y. ;

Levine, D. A. ;

Mills, G. B. ;

Morrison, C. ;

Mutch, D. ;

Olvera, N. ;

Orsulic, S. ;

Park, K. ;

Petrelli, N. ;

Rabeno, B. ;

Rader, J. S. ;

Sikic, B. I. ;

Smith-McCune, K. ;

Sood, A. K. ;

Bowtell, D. ;

Penny, R. ;

Testa, J. R. ;

Chang, K. ;

Dinh, H. H. ;

Drummond, J. A. ;

Fowler, G. ;

Gunaratne, P. ;

Hawes, A. C. ;

Kovar, C. L. ;

Lewis, L. R. ;

Morgan, M. B. ;

Newsham, I. F. ;

Santibanez, J. ;

Reid, J. G. ;

Trevino, L. R. ;

Wu, Y. -Q. ;

Wang, M. .

NATURE, 2011, 474 (7353) :609-615

[3] The CDK inhibitors in cancer research and therapy [J].

Cicenas, Jonas ;

Valius, Mindaugas .

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2011, 137 (10) :1409-1418

[4] Newly diagnosed and relapsed epithelial ovarian carcinoma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up [J].

Colombo, N. ;

Peiretti, M. ;

Parma, G. ;

Lapresa, M. ;

Mancari, R. ;

Carinelli, S. ;

Sessa, C. ;

Castiglione, M. .

ANNALS OF ONCOLOGY, 2010, 21 :v23-v30

[5] Cell Cycle Control of Wnt Receptor Activation [J].

Davidson, Gary ;

Shen, Jinlong ;

Huang, Ya-Lin ;

Su, Yi ;

Karaulanov, Emil ;

Bartscherer, Kerstin ;

Hassler, Christine ;

Stannek, Peter ;

Boutros, Michael ;

Niehrs, Christof .

DEVELOPMENTAL CELL, 2009, 17 (06) :788-799

[6] CDK14 Contributes to Reactive Gliosis via Interaction with Cyclin Y in Rat Model of Spinal Cord Injury [J].

Duan, Chengwei ;

Liu, Yonghua ;

Lu, Lu ;

Cai, Rixin ;

Xue, Huaqing ;

Mao, Xingxing ;

Chen, Chen ;

Qian, Rong ;

Zhang, Dongmei ;

Shen, Aiguo .

JOURNAL OF MOLECULAR NEUROSCIENCE, 2015, 57 (04) :571-579

[7] Upregulated PFTK1 promotes tumor cell proliferation, migration, and invasion in breast cancer [J].

Gu, Xiaoling ;

Wang, Yingying ;

Wang, Hua ;

Ni, Qichao ;

Zhang, Chunhui ;

Zhu, Jia ;

Huang, Wei ;

Xu, Pan ;

Mao, Guoxin ;

Yang, Shuyun .

MEDICAL ONCOLOGY, 2015, 32 (07)

[8] Cyclin Y, a novel membrane-associated cyclin, interacts with PFTK1 [J].

Jiang, Mei ;

Gao, Yankun ;

Yang, Tao ;

Zhu, Xueliang ;

Chen, Jiangye .

FEBS LETTERS, 2009, 583 (13) :2171-2178

[9] Newly diagnosed and relapsed epithelial ovarian carcinoma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up [J].

Ledermann, J. A. ;

Raja, F. A. ;

Fotopoulou, C. ;

Gonzalez-Martin, A. ;

Colombo, N. ;

Sessa, C. .

ANNALS OF ONCOLOGY, 2013, 24 :24-32

[10] Cyclin Y Is a Novel Conserved Cyclin Essential for Development in Drosophila [J].

Liu, Dongmei ;

Finley, Russell L., Jr. .

GENETICS, 2010, 184 (04) :1025-U232

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