Upregulation of SGLT-1 transport activity in rat jejunum induced by GLP-2 infusion in vivo

被引:208
作者
Cheeseman, CI [1 ]
机构
[1] Univ Alberta, Dept Physiol, Membrane Transport Grp, Edmonton, AB T6G 2H7, Canada
关键词
sodium-dependent glucose transporter; glucagon-like peptide-2; membrane transport; protein trafficking;
D O I
10.1152/ajpregu.1997.273.6.R1965
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The effect of in vivo infusion of the peptide hormone glucagon-like peptide 2 (GLP-2) on glucose transport across the rat jejunal brush-border membrane (BBM) was assessed using isolated membrane vesicles. A 2-h infusion of GLP-2 produced a marked acceleration of sodium-dependent glucose uptake into BBM vesicles with a significant overshoot. There was no change in vesicle space or permeability resulting from the hormone infusion. Kinetic analysis showed this stimulation to be the result of a threefold increase in the maximal rate of transport, with no consistent change in the affinity constant (K-m). The time course of this response showed that the effect was observable, but smaller, after only 30 min of hormone infusion and was maximal after 1 h. Sodium-dependent phloridzin binding to the membrane vesicles showed a parallel increase in maximal binding after 1 and 2 h of hormone infusion. Western blotting showed a similar increase in sodium-dependent glucose transporter 1 (SGLT-1) abundance. The effect of GLP-2 could be blocked by luminal brefeldin A or wortmannin. These results indicate that GLP-2 is able to induce trafficking of SGLT-1 from an intracellular pool into the BBM within 60 min and that phosphoinositol 3-kinase may well be involved in the intracellular signaling pathway in this response.
引用
收藏
页码:R1965 / R1971
页数:7
相关论文
共 30 条
[1]   BASOLATERAL TO APICAL TRANSCYTOSIS IN POLARIZED CELLS IS INDIRECT AND INVOLVES BFA AND TRIMERIC-G PROTEIN SENSITIVE PASSAGE THROUGH THE APICAL ENDOSOME [J].
BARROSO, M ;
SZTUL, ES .
JOURNAL OF CELL BIOLOGY, 1994, 124 (1-2) :83-100
[2]   HAMSTER PREPROGLUCAGON CONTAINS THE SEQUENCE OF GLUCAGON AND 2 RELATED PEPTIDES [J].
BELL, GI ;
SANTERRE, RF ;
MULLENBACH, GT .
NATURE, 1983, 302 (5910) :716-718
[3]  
BURANT CF, 1992, J BIOL CHEM, V267, P14523
[4]   GLUT2 IS THE TRANSPORTER FOR FRUCTOSE ACROSS THE RAT INTESTINAL BASOLATERAL MEMBRANE [J].
CHEESEMAN, CI .
GASTROENTEROLOGY, 1993, 105 (04) :1050-1056
[5]   The effect of GIP and glucagon-like peptides on intestinal basolateral membrane hexose transport [J].
Cheeseman, CI ;
Tsang, R .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1996, 271 (03) :G477-G482
[6]   ADAPTATION OF GLUCOSE-TRANSPORT ACROSS RAT ENTEROCYTE BASOLATERAL MEMBRANE IN RESPONSE TO ALTERED DIETARY CARBOHYDRATE INTAKE [J].
CHEESEMAN, CI ;
HARLEY, B .
JOURNAL OF PHYSIOLOGY-LONDON, 1991, 437 :563-575
[7]   RAPID REGULATION OF D-GLUCOSE TRANSPORT IN BASOLATERAL MEMBRANE OF RAT JEJUNUM [J].
CHEESEMAN, CI ;
MAENZ, DD .
AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 256 (05) :G878-G883
[8]   ACUTE AND CHRONIC EFFECTS OF PANCREATIC GLUCAGON ON SUGAR-TRANSPORT ACROSS THE BRUSH-BORDER AND BASOLATERAL MEMBRANES OF RAT JEJUNAL ENTEROCYTES [J].
DEBNAM, ES ;
SHARP, PA .
EXPERIMENTAL PHYSIOLOGY, 1993, 78 (02) :197-207
[9]   Effect of luminal epidermal growth factor on enterocyte glucose and proline transport [J].
Hardin, JA ;
Wong, JK ;
Cheeseman, CI ;
Gall, DG .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1996, 271 (03) :G509-G515
[10]   EXPRESSION CLONING AND CDNA SEQUENCING OF THE NA+/GLUCOSE COTRANSPORTER [J].
HEDIGER, MA ;
COADY, MJ ;
IKEDA, TS ;
WRIGHT, EM .
NATURE, 1987, 330 (6146) :379-381