GENERATION AND CHARACTERIZATION OF 11 NOVEL EST DERIVED MICROSATELLITES FROM VICIA FABA (FABACEAE)

被引：27

作者：

Gong, Ya-Ming ^{[1
]}

Xu, Sheng-Chun ^{[1
]}

Mao, Wei-Hua ^{[2
]}

Hu, Qi-Zan ^{[1
]}

Zhang, Gu-Wen ^{[1
]}

Ding, Ju ^{[1
]}

Li, Ze-Yun ^{[2
]}

机构：

[1] Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China

[2] Zhejiang Univ, Ctr Anal & Measurement, Hangzhou 310029, Zhejiang, Peoples R China

来源：

AMERICAN JOURNAL OF BOTANY | 2010年 / 97卷 / 07期

关键词：

expressed sequence tag (EST); faba bean (Vicia faba L.); microsatellite; transferability; MARKERS; SSRS;

D O I：

10.3732/ajb.1000166

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Simple sequence repeat (SSR) markers were developed for faba bean using expressed sequence tags (ESTs) from the NCBI database to study for genetic diversity. Methods and Results: A total of 11 novel EST-SSR loci were generated and characterized when tested on four populations of 29 faba bean individuals from China and Europe. The number of alleles (A) ranged from 1 to 3 in each population, and observed heterozygosity (H(O)) and expected heterozygosity (H(E)) ranged from 0 to 0.5000 and 0.6400, respectively. Furthermore, transferable analysis revealed that eight of these loci (72.73%) amplified in Pisum sativum L., six of which (75.00%) detected polymorphism. Conclusions: The developed markers in this study will provide valuable tools for genetic diversity, resource conservation, genetic mapping, and marker-assisted breeding of faba bean in the future.

引用

页码：E69 / E71

页数：3

共 8 条

[1] Diversity maintenance and use of Vicia faba L. genetic resources
Duc, Gerard
Bao, Shiying
Baum, Michael
Redden, Bob
Sadiki, Mohammed
Jose Suso, Maria
Vishniakova, Margarita
Zong, Xuxiao
[J]. FIELD CROPS RESEARCH, 2010, 115 (03) : 270 - 278
[2] EST-SSRs as a resource for population genetic analyses
Ellis, J. R.
Burke, J. M.
[J]. HEREDITY, 2007, 99 (02) : 125 - 132
[3] Development and characterization of microsatellite markers from chromosome 1-specific DNA libraries of Vicia faba
Pozárková, D
Koblizková, A
Román, B
Torres, AM
Lucretti, S
Lysák, M
Dolezel, J
Macas, J
[J]. BIOLOGIA PLANTARUM, 2002, 45 (03) : 337 - 345
[4] Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers
Terzopoulos, P. J.
Bebeli, P. J.
[J]. FIELD CROPS RESEARCH, 2008, 108 (01) : 39 - 44
[5] Genic microsatellite markers in plants: features and applications
Varshney, RK
Graner, A
Sorrells, ME
[J]. TRENDS IN BIOTECHNOLOGY, 2005, 23 (01) : 48 - 55
[6] Yeh FC., 1999, POPGENE
[7] Simple sequence repeats (SSRs) in faba bean: new loci from Orobanche-resistant cultivar 'Giza 402'
Zeid, M.
Mitchell, S.
Link, W.
Carter, M.
Nawar, A.
Fulton, T.
Kresovich, S.
[J]. PLANT BREEDING, 2009, 128 (02) : 149 - 155
[8] Molecular variation among Chinese and global winter faba bean germplasm
Zong, Xuxiao
Liu, Xiuju
Guan, Jianping
Wang, Shumin
Liu, Qingchang
Paull, Jeffrey G.
Redden, Robert
[J]. THEORETICAL AND APPLIED GENETICS, 2009, 118 (05) : 971 - 978

← 1 →