Rapid development of neutralizing and diagnostic SARS-COV-2 mouse monoclonal antibodies

被引：14

作者：

Chapman, Asheley P. ^{[1
]}

Tang, Xiaoling ^{[2
]}

Lee, Joo R. ^{[2
]}

Chida, Asiya ^{[2
]}

Mercer, Kristina ^{[3
]}

Wharton, Rebekah E. ^{[3
]}

Kainulainen, Markus ^{[4
]}

Harcourt, Jennifer L. ^{[5
]}

Martines, Roosecelis B. ^{[6
]}

Schroeder, Michelle ^{[1
]}

Zhao, Liangjun ^{[1
]}

Bryksin, Anton ^{[7
]}

Zhou, Bin ^{[8
]}

Bergeron, Eric ^{[4
]}

Bollweg, Brigid C. ^{[6
]}

Tamin, Azaibi ^{[5
]}

Thornburg, Natalie ^{[5
]}

Wentworth, David E. ^{[8
]}

Petway, David ^{[2
]}

Bagarozzi, Dennis, Jr. ^{[2
]}

Finn, M. G. ^{[1
,9
]}

Goldstein, Jason M. ^{[2
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, 901 Atlantic Dr, Atlanta, GA 30306 USA

[2] Reagent Diagnost Serv Branch RDSB DSR NCEZID CDC, Immunodiagnost Dev Team, 1600 Clifton Rd NE, Atlanta, GA 30333 USA

[3] Div Lab Sci DLS NCEH CDC, 4770 Buford Hwy, Atlanta, GA 30341 USA

[4] Viral Special Pathogens Branch VSPB DHCPP NCEZID, 1600 Clifton Rd NE, Atlanta, GA 30333 USA

[5] Resp Dis Branch RDB DVD NCIRD CDC, 1600 Clifton Rd NE, Atlanta, GA 30333 USA

[6] Infect Dis Pathol Branch IDPB DHCPP NCEZID CDC, 1600 Clifton Rd NE, Atlanta, GA 30333 USA

[7] Georgia Inst Technol, Parker H Petit Inst Bioengn & Biosci, Atlanta, GA 30306 USA

[8] Virol Surveillance & Diag Branch VSPB ID NCIRD CD, Vaccine Preparedness Team, 1600 Clifton Rd NE, Atlanta, GA 30333 USA

[9] Georgia Inst Technol, Sch Biol Sci, 901 Atlantic Dr, Atlanta, GA 30306 USA

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

SARS CORONAVIRUS; DENGUE VIRUS; RECEPTOR; PROTEIN; EXPRESSION; INFECTION; KINETICS; IMMUNITY; BINDING; FUSION;

D O I：

10.1038/s41598-021-88809-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of the global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest is the- 300 amino acid receptor binding domain (RBD) within the S1 subunit of the spike protein because of its key interaction with the human angiotensin converting enzyme 2 (hACE2) receptor present on many cell types, especially lung epithelial cells. We report here the development and functional characterization of 29 nM-affinity mouse SARS-CoV-2 mAbs created by an accelerated immunization and hybridoma screening process. Differing functions, including binding of diverse protein epitopes, viral neutralization, impact on RBD-hACE2 binding, and immunohistochemical staining of infected lung tissue, were correlated with variable gene usage and sequence.

引用

页数：12

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