Phospholipase C-γ1 is required for the epidermal growth factor receptor-induced squamous cell carcinoma cell mitogenesis

被引：36

作者：

Xie, Zhongjian ^{[1
,3
]}

Chen, Ying ^{[2
]}

Liao, Er-Yuan ^{[3
]}

Jiang, Yi ^{[4
]}

Liu, Fu-You ^{[5
]}

Pennypacker, Sally D. ^{[1
]}

机构：

[1] Univ Calif San Francisco, No Calif Inst Res & Educ, Vet Affairs Med Ctr, Endocrine Unit, San Francisco, CA 94121 USA

[2] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

[3] Cent S Univ, Xiangya Hosp 2, Inst Metab & Endocrinol, Changsha 410011, Hunan, Peoples R China

[4] Cent S Univ, Xiangya Hosp 2, Dept Pathol, Changsha 410011, Hunan, Peoples R China

[5] Cent S Univ, Xiangya Hosp 2, Res Inst Nephrol, Div Nephrol, Changsha 410011, Hunan, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 397卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Epidermal growth factor receptor; Phospholipase C-gamma 1; Mitogenesis; Squamous cell carcinoma; HUMAN KERATINOCYTE DIFFERENTIATION; EGF RECEPTOR; ELEVATED CONTENT; C ACTIVITY; ACTIVATION; IMMUNOREACTIVITY; OVEREXPRESSION; MESSENGER; MEMBRANE; INVASION;

D O I：

10.1016/j.bbrc.2010.05.103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The epidermal growth factor receptor (EGFR) is a key driver in the process of squamous cell carcinoma (SCC) cell mitogenesis. Phospholipase C-gamma 1 (PLC-gamma 1) is a downstream target of EGFR signaling, but the role and necessity of PLC-gamma 1 in EGFR-induced cell mitogenesis remain unclear. In the present study, we report an elevated expression of PLC-gamma 1 in human SCC biopsies relative to adjacent normal epidermis, and in human SCC cell lines compared to normal human keratinocytes. EGFR-induced SCC cell mitogenesis was blocked by small interfering RNA knockdown of PLC-gamma 1. However, inhibition of the catalytic activity of phospholipase C had no effect on EGFR-induced SCC cell mitogenesis. In response to the EGFR ligand epidermal growth factor (EGF), PLC-gamma 1 was translocated not only to the plasma membrane but also to the nucleus. These data suggest that PLC-gamma 1 is required for EGFR-induced SCC cell mitogenesis and the mitogenic function of PLC-gamma 1 is independent of its lipase activity. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：296 / 300

页数：5

共 31 条

[1] ELEVATED CONTENT OF THE TYROSINE KINASE SUBSTRATE PHOSPHOLIPASE C-GAMMA-1 IN PRIMARY HUMAN BREAST CARCINOMAS [J].