Puffer fish-based commercial fraud identification in a segment of cytochrome b region by PCR-RFLP analysis

被引：41

作者：

Hsieh, Cheng-Hong ^{[1
]}

Chang, Wen-Teish ^{[2
]}

Chang, Hebron C. ^{[3
]}

Hsieh, Hung-Sheng ^{[1
]}

Chung, Yun-Lung ^{[1
]}

Hwang, Deng-Fwu ^{[4
]}

机构：

[1] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 41354, Taiwan

[2] Natl Penghu Univ, Dept Food Sci, Penghu, Taiwan

[3] Asia Univ, Dept Biotechnol, Taichung 41354, Taiwan

[4] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung, Taiwan

来源：

FOOD CHEMISTRY | 2010年 / 121卷 / 04期

关键词：

Food authenticity; Species identification; Puffer fish-based product; PCR-RFLP; Thermal treatment product; Dry-dressed fish fillet; Cytochrome b gene; POLYMERASE-CHAIN-REACTION; SPECIES IDENTIFICATION; MITOCHONDRIAL-DNA; PHYLOGENETIC-RELATIONSHIPS; GENETIC IDENTIFICATION; REACTION PRODUCTS; RESTRICTION SITE; SEQUENCE; TETRODOTOXIN; EVOLUTION;

D O I：

10.1016/j.foodchem.2010.02.004

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To identify the mislabeled or fraudulently substituted toxic puffer fish in thermally processed fish products, a polymerase chain reaction (PCR) method using restriction sites and sequence analysis has been developed in this study. A 376-bp fragment of the cytochrome b gene was produced after PCR amplification. Fish tissue samples were prepared under autoclaving conditions at 121 degrees C for 10-90 min at 10 min intervals. DNA fragments could not be detected after 90 min of autoclaving at 121 C. For PCR product digestion, BsaJ I, Aci I, Hinf I. Taq I, and Sap I endonucleases were used to yield species-specific profiles for the identification of puffer fish species from 60 commercial market samples. Results from this study showed that the restriction fragment length polymorphism technique can be used to identify 17 puffer fish species from commercial products even after severe thermal processing. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1305 / 1311

页数：7

共 30 条

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