Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

被引：9

作者：

Chen, Song ^{[1
]}

Hou, Peng ^{[1
]}

Sun, Jingwen ^{[1
]}

Wang, Haijun ^{[1
]}

Liu, Lei ^{[1
]}

机构：

[1] Qiqihar Med Univ, Coll Pharm, Qiqihar 161006, Peoples R China

来源：

MOLECULES | 2019年 / 24卷 / 18期

基金：

黑龙江省自然科学基金;

关键词：

large Stokes shift; zebrafish; thiols; cell imaging; imidazo[1; 5-alpha]pyridine; LARGE STOKES SHIFT; FLUORESCENT-PROBE; SELECTIVE DETECTION; CYSTEINE; GLUTATHIONE; SENSOR; HOMOCYSTEINE; ROLES;

D O I：

10.3390/molecules24183328

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-alpha]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.

引用

页数：9

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