A new substrate to measure laccase activities in complex environments: Application to litters

被引：12
作者：
Farnet, A. M. [1 ]
Ferre, E. [1 ]
Gil, G. [2 ]
Gastaldi, S. [3 ]
机构：
[1] Univ Paul Cezanne, Fac Sci & Tech St Jerome, UMR CNRS IRD 6116, Inst Mediterraneen Ecol & Paleoecol,Equipe Ecol M, F-13397 Marseille 20, France
[2] Univ Paul Cezanne, Fac Sci & Tech St Jerome, UMR CNRS 6263, Equipe Chirosci, F-13397 Marseille 20, France
[3] Univ Paul Cezanne, Fac Sci St Jerome, LCP UMR 6264, Equipe Chim Mol Organ, F-13397 Marseille 20, France
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 2010年 / 42卷 / 06期
关键词：
Abiotic interaction; Phenoloxidase; Syringaldazine analogue; Litter; MARASMIUS-QUERCOPHILUS; ANTIOXIDANT CAPACITY; PLEUROTUS-OSTREATUS; FUNGAL LACCASE; LEAF-LITTER; DEGRADATION; MEDIATORS; ASSAY; TEAC; ACID;
D O I：
10.1016/j.soilbio.2010.02.005
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
Measuring laccase activities in natural environments requires improvement since this oxidation usually produces radical compounds which can interfere with other molecules from these complex media, leading to misinterpretation of the results. Here the syringaldazine skeleton was used to synthesise a new substrate whose quinone is soluble in water and chemically stable. Hydrophilic moieties were added to syringaldazine at the 3 and 5 positions on both aromatic rings to enhance its solubility in water. Syringaldazine analogue 6c was selected. Laccases from two white-rot fungus strains were first tested and a strong affinity for this substrate was observed. Moreover the laccase activity found using a litter extract was higher with 6c which can be explained by the strong stability of its quinone in water. (C) 2010 Elsevier Ltd. All rights reserved.
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收藏
页码：1001 / 1005
页数：5
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