Mouse model of skeletal muscle adiposity: A glycerol treatment approach

被引：53
作者：
Pisani, Didier F. [1 ]
Bottema, Cynthia D. K. [2 ]
Butori, Catherine [3 ]
Dani, Christian
Dechesne, Claude A.
机构：
[1] Univ Nice Sophia Antipolis, Fac Med,CNRS, UMR6543, Inst Dev Biol & Canc,ISBDC, F-06107 Nice 2, France
[2] Univ Adelaide, Sch Anim & Vet Sci, Roseworthy, SA 5371, Australia
[3] Hop Louis Pasteur, CHU Nice, Lab Clin & Expt Pathol, F-06002 Nice, France
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 396卷 / 03期
关键词：
Adipocyte; White adipose tissue; Muscle regeneration; Myonecrosis; SARCOGLYCAN-DEFICIENT MICE; MUSCULAR-DYSTROPHIES; REGENERATION; MACROPHAGES; DISEASES; INJURY; ALPHA;
D O I：
10.1016/j.bbrc.2010.05.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fat cell accumulation in skeletal muscle is a major characteristic of various disorders, such as obesity, sarcopenia and dystrophies. Moreover, these fat cells could be involved in muscle homeostasis regulation as previously described for adipocytes in bone marrow. Despite recent advances on the topic, no clearly characterized mouse model is currently available to study fat accumulation within skeletal muscle. Here, we report a detailed characterization of a mouse model of skeletal muscle fat cell accumulation after degeneration induced by intra-muscular injection of glycerol. Information is provided on the kinetics of degeneration/fat deposition, including the quantity of fat deposited based on various parameters such as glycerol concentration, age, sex and strain of mice. Finally, these fat cells are characterized as true white adipocytes morphologically and molecularly. Our study shows that the mouse adipocyte accumulation within skeletal muscle after glycerol degeneration is a reproducible, transposable and easy model to use. This mouse model should allow a more comprehensive understanding of the impact of adipocyte accumulation in skeletal muscle pathophysiology. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：767 / 773
页数：7
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