Expression of bmp2a and bmp2b in late-stage zebrafish median fin development

被引:22
作者
Crotwell, PL [1 ]
Sommervold, AR [1 ]
Mabee, PM [1 ]
机构
[1] Univ S Dakota, Dept Biol, Vermillion, SD 57069 USA
基金
美国国家科学基金会;
关键词
zebrafish; bmp2; bmp2a; bmp2b; median fins; radials; hypurals; fin rays; neural and hemal arches; neural and hemal spines; lens; cartilage; segmentation; RT-PCR; in situ hybridization;
D O I
10.1016/j.modgep.2004.07.001
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Zebrafish bmp2a and bmp2b mRNA expression in the developing median fins (caudal, anal, and dorsal) of late-stage larvae (> 5 days post-fertilization) was analyzed by reverse transcriptase-PCR (RT-PCR) and in situ hybridization. bmp2a is expressed in developing fin rays, while bmp2b is expressed in developing fin rays, hypertrophic chondrocytes, and in the zone of segmentation (ZS) in developing anal and dorsal fin radials. This latter pattern of bmp2b expression in the ZS mirrors tetrapod bmp2 expression in developing joints. Additionally, both genes are expressed in neural and hemal arches and spines. bmp2a is strongly expressed in the lens; lens bmp2b expression is detected only weakly via RT-PCR. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:291 / 296
页数:6
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