Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production

被引：11

作者：

Guimaraes de Oliveira, Louise Helena ^{[1
]}

Paiva Silva de Sousa, Patricia Alexandria ^{[1
]}

Hilario, Fabrine Felipe ^{[1
]}

Nascimento, Gabriel Joventino ^{[1
]}

Saraiva Morais, Joao Paulo ^{[2
]}

de Medeiros, Everaldo Paulo ^{[2
]}

de Sousa, Manoel Francisco ^{[2
]}

Nunes, Fabiola da Cruz ^{[1
]}

机构：

[1] Univ Fed Paraiba, Ctr Biotechnol, BR-58051900 Joao Pessoa, Paraiba, Brazil

[2] Brazilian Agr Res Corp, Embrapa Algodao, Campina Grande, PB, Brazil

来源：

ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE | 2016年 / 6卷 / 05期

关键词：

Cell culture; Insecticide; Mosquito; Sisal;

D O I：

10.1016/j.apjtb.2015.12.018

中图分类号：

R188.11 [热带医学];

学科分类号：

摘要：

Objective: To investigate the effects of Agave sisalana (A. sisalana) extract on Aedes aegypti (Ae. aegypti) primary cell culture. Methods: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract (0.18-6.00 mg/mL) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was measured. Results: Results showed that cells exposed to 6 mg/mL of the crude extract presented a greater percentage of death when compared to control (73.8% +/- 9.6% vs. 34.6% +/- 9.6%). Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/ mL of A. sisalana crude extract [(0.81 +/- 0.08) mmol/ L] compared to control group [(0.41 +/- 0.18) mmol/ L]. Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber.

引用

页码：396 / 399

页数：4

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