Germination of Bacillus cereus spores adhered to stainless steel

被引:43
|
作者
Hornstra, L. M.
de Leeuw, P. L. A.
Moezelaar, R.
Wolbert, E. J.
de Vries, Y. P.
de Vos, W. M.
Abee, T.
机构
[1] Univ Wageningen & Res Ctr, Lab Food Microbiol, NL-6703 HD Wageningen, Netherlands
[2] Wageningen Ctr Food Sci, Wageningen, Netherlands
关键词
Bacillus cereus; germination; CIP; adhesion;
D O I
10.1016/j.ijfoodmicro.2007.02.012
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Adhered spores of Bacillus cereus represent a significant part of the surface-derived contamination in processing equipment used in the dairy industry. As germinated spores lose their resistance capacities instantaneously, efficient germination prior to a cleaning in place treatment could aid to the disinfecting effect of such a treatment. Therefore, spores of B. cereus ATCC 14579 and that of the environmental isolate B. cereus CMCC 3328 were assessed for their germination behaviour when adhered to a stainless steel surface. A mixture of I-alanine and inosine initiated germination of adhered spores efficiently, resulting in 3.2 decimal logarithms of germination. Notably, implementation of a germination-inducing step prior to a representative cleaning in place procedure reduced the number of survivors with over 3 decimal log units, while an alkali treatment alone, as part of the cleaning in place procedure, did not show any effect on B. cereus spore viability. These results show that implementation of a germination step enhances the disinfection effect of currently used cleaning in place procedures. (c) 2007 Published by Elsevier B.V.
引用
收藏
页码:367 / 371
页数:5
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