Fungi isolated from insects in strawberry crops act as potential biological control agents of Duponchelia fovealis (Lepidoptera: Crambidae)

被引:29
作者
Poitevin, Carolina Gracia [1 ]
Porsani, Mariana Vieira [1 ]
Poltronieri, Alex Sandro [2 ]
Cassilha Zawadneak, Maria Aparecida [3 ]
Pimentel, Ida Chapaval [1 ]
机构
[1] Univ Fed Parana, Dept Basic Pathol, Lab Microbiol & Mol Biol LabMicro, Av Cel Francisco H dos Santos S-N, BR-81531980 Curitiba, Parana, Brazil
[2] Univ Fed Santa Catarina, Dept Plant Sci, Agr Sci Ctr, Rodovia Admar Gonzaga 1346, BR-88034000 Florianopolis, SC, Brazil
[3] Univ Fed Parana, Dept Basic Pathol, Lab Entomol Prof Angelo Moreira de Costa Lima, Av Cel Francisco H dos Santos S-N, BR-81531980 Curitiba, Parana, Brazil
关键词
Entomopathogenic fungi; Beauveria bassiana; Fragaria x ananassa; European pepper moth; ENTOMOPATHOGENIC FUNGI; BEAUVERIA-BASSIANA; METARHIZIUM-ANISOPLIAE; PATHOGENICITY; SOILS; BIOCONTROL; DIVERSITY; VIRULENCE; SELECTION; SAFETY;
D O I
10.1007/s13355-018-0561-0
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Entomopathogenic fungi were collected from insects, belonging to orders Lepidoptera, Coleoptera, Hemiptera, Hymenoptera, and Diptera, with signs of infection. Single-spore colonies were identified based on morphological traits and ribosomal ITS sequences. The most commonly found fungi were Fusarium oxysporum Schlechtendal and Beauveria bassiana (Balsamo-Crivelli) Vuillemin. Isolation of Trichoderma atroviride Bisset, Aspergillus flavipes (Bainier and Sartory) Thom and Church, Aspergillus iizukae Sugiyama, Penicillium mallochii Rivera, Urb and Seifert, Penicillium adametzioides S. Abe ex G. Smith, and Mucor nidicola Madden, Stchigel, Guarro and Starks associated to insects is reported for the first time. Tests with B. bassiana isolates against Duponchelia fovealis Zeller (Lepidoptera: Crambidae), showed high larval mortality in vitro and in greenhouse, demonstrating its potential as biological control agent. Understanding the fungal microbiota from insects can provide promising isolates for use in integrated pest management programs. This is the first report characterizing the mycobiota in insects collected in strawberry crops, and evaluating the pathogenicity of B. bassiana against D. fovealis, a pest that causes severe losses to farmers.
引用
收藏
页码:323 / 331
页数:9
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